Name: Zika Virus
Test Name: Zika Virus
Health Link Test Code: Zika information
LIS Test Code: Zika information
Testing Site: Testing will be sent to an approved reference laboratory
All testing must be preapproved by Wisconsin Public Health.
1) All requests for Zika Virus must be approved by the Wisconsin Department of Public Health (WDPH) before submitting specimens to WSLH.
2) BEFORE COLLECTION of specimen(s) in a suspected case of Zika Virus, ordering provider must contact WDPH at (608) 267-9003. The Wisconsin State Laboratory of Hygiene (WSLH) is now able to perform testing upon approval by the WDPH.
3) Once approved, WDPH will fax approval form to the ordering provider.
4) Ordering provider will place orders in Healthlink.
5) Give patient a copy of the approval form and send them to have their blood collected.
6) Approval form must be presented at the time of collection and accompany blood to the UWHC lab.
7) Contact ordering provider if patient presents without the approval form.
8) UWHC lab will inform WSLH a specimen will be submitted.
WSLH will test or forward specimen to CDC for testing as follows:
● Specimen is collected within 3 days of symptom onset – Order Arbovirus, PCR (ARBOPCR) - the WSLH will perform PCR testing for Zika, Dengue and Chikungunya viruses on serum specimens collected within 3 days of symptom onset. Urine specimens may also be submitted for testing, but they will only be tested if a serum specimen is also submitted.
● Specimen is collected 4-7 days after symptom onset – Order Arbovirus, PCR (ARBOPCR) and Zika Virus Ab, IgM (ZIKAIGM) - the WSLH will perform the PCR testing for Zika, Dengue and Chikungunya as above and will add serum IgM testing for Zika virus. Serum IgM testing for Dengue and Chikungunya will continue to be sent to the CDC.
● Specimen is collected 1- 12 weeks after symptom onset – Order Zika Virus Ab, IgM (ZIKAIGM) - the WSLH will perform serum IgM testing for Zika virus. Serum IgM testing for Dengue and Chikungunya will continue to be sent to the CDC.
● Specimen is collected 2-12 weeks after travel to an endemic area from asymptomatic pregnant women - Order Zika Virus Ab, IgM (ZIKAIGM) – the WSLH will only perform serum IgM testing for Zika Virus.
● Specimens testing positive or equivocal for Zika virus IgM – the WSLH will forward specimens to the CDC for confirmatory plaque-reduction neutralization (PRNT) testing.
Specimen: A minimum of 1.0 mL of serum or CSF on a cold pack.
Additional information on Zika Virus infections can be found at: