Name: M. tuberculosis PCR
Test Name: M. tuberculosis PCR
Health Link Test Code: TBPCR
LIS Test Code: TBPCR
CPT Code(s): 87556
This test detects the M. tuberculosis complex. This test is always performed on first smear positive specimens submitted to WSLH.
Testing Site: Testing will be sent to an approved reference laboratory
Specimen: See collection instructions.
Collection Container: State Lab Acid Fast Bacillus (AFB) collection kit
Also Acceptable: Sterile screw cap container
Submit 3-10 mL of a primary respiratory specimen (sputum, tracheal aspirate, brochial washing, BAL).
For primary, non-respiratory specimens (body fluids, CSF, stool, urine, lung and other tissues):
Stool: 1 g in sterile container
Tissue Biopsy: 1 g in sterile container
Urine: 40 mL, first-morning, clean catch in sterile container (24-hour pooled specimen is unacceptable)
Body Fluids (pleural, pericardial, etc.): 5-10mL in sterile container
Whole Blood: 10 mL in SPS blood collection tube or Heparin tube (do not use EDTA)
Bone Marrow: As much as available in sterile container or SPS tube
CSF: As much as available in sterile container (minimum of 1 mL required)
Skin Lesions: scrapings or exudates; swabs are not recommended.
Specimens with visible blood will be accepted. All specimens should be submitted in sterile, leak-proof containers.
TB PCR testing must be performed within 7 days of specimen collection.
Transport specimen to UWHC Hospital Laboratory. Transport with coolant pack if coming from clinic location.
Specimens from patients who have undergone antimicrobial therapy for TB for more than 7 days in the past 12 months. Specimens from patients for whom suspicion of TB is low.
This tests detects M. tuberculosis complex but does not differentiate among its members.
AFB smear and culture must also be ordered on any specimen submitted for PCR testing.