Nov 23, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,WSLH

M. tuberculosis PCR

M. tuberculosis PCR (TBPCR) WSLH Lab Test

Name: M. tuberculosis PCR

Test Name: M. tuberculosis PCR

Health Link Test Code: TBPCR

LIS Test Code: TBPCR

CPT Code(s): 87556

Clinical Information:

This test detects the M. tuberculosis complex. This test is always performed on first smear positive specimens submitted to WSLH.

Testing Site: Testing will be sent to an approved reference laboratory

Specimen: See collection instructions.

Collection Container: State Lab Acid Fast Bacillus (AFB) collection kit

Also Acceptable: Sterile screw cap container

Collection Instructions:

Submit 3-10 mL of a primary respiratory specimen (sputum, tracheal aspirate, brochial washing, BAL).


For primary, non-respiratory specimens (body fluids, CSF, stool, urine, lung and other tissues):

Stool: 1 g in sterile container

Tissue Biopsy: 1 g in sterile container

Urine: 40 mL, first-morning, clean catch in sterile container (24-hour pooled specimen is unacceptable)

Body Fluids (pleural, pericardial, etc.): 5-10mL in sterile container

Whole Blood: 10 mL in SPS blood collection tube or Heparin tube (do not use EDTA)

Bone Marrow: As much as available in sterile container or SPS tube

CSF: As much as available in sterile container (minimum of 1 mL required)

Skin Lesions: scrapings or exudates; swabs are not recommended.


Specimens with visible blood will be accepted. All specimens should be submitted in sterile, leak-proof containers.

Stability Refridgerated:

7 days

Stability Frozen:

Not acceptable

Specimen Processing:

TB PCR testing must be performed within 7 days of specimen collection.

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Specimens from patients who have undergone antimicrobial therapy for TB for more than 7 days in the past 12 months. Specimens from patients for whom suspicion of TB is low.

Test Limitations:

This tests detects M. tuberculosis complex but does not differentiate among its members.

Additional Information:

AFB smear and culture must also be ordered on any specimen submitted for PCR testing.