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Nov 23, 2015

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100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,WSLH

AFB Smear and Culture

AFB Smear and Culture (AFBSMR/AFBCX; AFB Isolation order panel (inpatients)) WSLH Lab Test

Name: AFB Smear and Culture

Test Name: AFB Smear and Culture

Health Link Test Code: AFBSMR/AFBCX; AFB Isolation order panel (inpatients)

LIS Test Code: AFBCX and AFBSMR

Testing Site: Testing will be sent to an approved reference laboratory

Link: http://www.slh.wisc.edu/wslhApps/RefMan/wslhSearch.php?searchTerm=afb&TEST_REFERENCE_ID=5476&submitIt=testDetail

Specimen: See collection instructions.

Collection Instructions:

 

Specimen Container Instructions/Comments
Blood

Preferred: Yellow top (w/ SPS Anticoagulant)

Also acceptable: Green top (w/ NaHeparin)

Smear not performed on blood specimens due to very low bacterial contamination.
Bone Marrow

Preferred:  Yellow top (w/ SPS Anticoagulant)

Also acceptable: Green top (w/ Na Heparin)

 
Body Fluid, CSF

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit (CS item# 2200232).
Gastric Lavage SLH Gastric collection kit

Collect fasting gastric lavage soon after patient awakens to obtain sputum swallowed during sleep.  

Immediately neutralize gastric lavage by placing in SLH Gastric collection kit containing buffer.  

Obtain collection kit from UWHC Core Lab, (608) 263-7060.

Collect 3 specimens in a series of 3-5 days.

Skin Lesion

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit
Sputum

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit (CS item# 2200232).  

Collect at least three consecutive sputum specimens, each collected in 8-24 hour intervals,

with at least one being an early morning specimen.

Stool

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit (CS item# 2200232).
Tissue

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit (CS item# 2200232).
Urine, Random

Preferred: SLH Acid Fast Bacillus (AFB) collecction kit

Also acceptable:  Sterile collection container

Follow packaging instructions included with kit (CS item# 2200232).  

Collect 40 mL first morning, clean catch in sterile container.

24-hr pooled specimens are not acceptable.

 

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory immediately. Transport with coolant pack if coming from clinic location.