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Apr 2, 2015

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Direct Antiglobulin Test, Transfusion Reaction Workup

Direct Antiglobulin Test, Transfusion Reaction Workup (HCDATR) Transfusion Service Lab Test

Name: Direct Antiglobulin Test, Transfusion Reaction Workup

Test Name: Direct Antiglobulin Test, Transfusion Reaction Workup

Health Link Test Code: HCDATR

LIS Test Code: DATR

CPT Code(s): 86880

Methodology: Hemagglutination

Days Performed: Daily, 24 hours.

In-Lab Turnaround Time: 8 hours.

Stat In-Lab Turnaround Time: 1 hour.

Specimen: Blood

Collection Container: Lavender top

Also Acceptable: Microtainer, Pink top tube

Collection Instructions:

Indicate collection date and time on specimen.  Testing can be performed on the same sample for the Transfusion Reaction ABO and Rh test.

Collection Volume: 4 mL

Pediatric Collection Volume: 0.5 mL

Stability Ambient:

24 hours

Stability Refridgerated:

24 - 48 hours

Stability Frozen:

Not acceptable

Sample Analyzed: Red cells

Specimen Transport:

Transport specimen to UWHC Transfusion Service or send to tube station 134.

Unacceptable Criteria:

Clotted specimens are not acceptable.

Interpretation:

Negative

Interpretation Type: Expected Results

Additional Information:

All red cells found to be positive with polyspecific Coombs' serum will be further tested with monospecific Coombs' sera (anti-IgG and anti-complement).