Aug 9, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Toxicology


Tobramycin (HCTOBR) Toxicology Lab Test

Name: Tobramycin

Test Name: Tobramycin

Health Link Test Code: HCTOBR

LIS Test Code: TOBR

CPT Code(s): 80200

Methodology: Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)

Clinical Information:


Days Performed: Daily, 24 hours.

In-Lab Turnaround Time: 4 hours.

Stat In-Lab Turnaround Time: 1 hour.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Green top (with lithium heparin anticoagulant)

Collection Instructions:

Collect specimen for peak level 30 minutes after the end of a 30 or 60 minute infusion. Collect specimen for trough level 15 - 30 minutes prior to start of infusion.

Collection Volume: 2 mL

Pediatric Collection Volume: 0.4 mL

Stability Refridgerated:

7 days

Stability Frozen:

14 days

Sample Analyzed: Serum and plasma

Testing Volume: 1 mL

Pediatric Testing Volume: 0.2 mL

Specimen Processing:

Centrifuge immediately. Transfer cell-free serum or plasma to plastic vial. Freeze.

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory within 30 minutes. Transport frozen serum on dry ice if coming from clinic location.

Unacceptable Criteria:

Specimens collected in red cap with yellow ring (SST) or green cap with yellow ring (PST) tubes are not acceptable. Specimens greater than 2 hours old, uncentrifuged when received by the laboratory, are not acceptable.


Optimal therapeutic range:

Peak: 5.0-10.0 ug/mL

Trough: less than 2.0 ug/mL

Interpretation Type: Interpretive Guidelines

Test Limitations:

Patient samples which contain amikacin, Kanamycin A, Kanamycin B, 3',4' dideoxykanamycin B will yield falsely elevated values for Tobramycin.

Critical Calls: yes

Additional Information:

Result of "BELOW" indicates that the result is below the method detection limit of 0.2 ug/mL. Tobramycin is an aminoglycoside antibiotic whose in-vitro activity is similar to that of gentamicin against gram-negative bacteria: it is more active than gentamicin against Pseudomonas aeruginosa. It also has activity against staphylococci.