Mar 24, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Toxicology

Nicotine (includes Cotinine)

Nicotine (includes Cotinine) (HCNCCPNL) Toxicology Lab Test

Name: Nicotine (includes Cotinine)

Test Name: Nicotine (includes Cotinine)

Health Link Test Code: HCNCCPNL


CPT Code(s): 80323

Methodology: Gas Chromatography/Mass Spectrometry

Days Performed: Mon-Fri, dayshift, as workload dictates.

In-Lab Turnaround Time: 4 - 7 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red top

Collection Volume: 4 mL

Pediatric Collection Volume: 3 mL

Stability Ambient:

7 days

Stability Refridgerated:

7 days

Stability Frozen:

1 year

Sample Analyzed: Serum

Testing Volume: 2 mL

Pediatric Testing Volume: 1.5 mL

Specimen Processing:

Centrifuge and transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.


Result of "BELOW DETECTION" indicates that the result is below the method detection limit of 50 ng/mL for nicotine and 50 ng/mL for cotinine.

Test Limitations:

Nicotine and cotinine will be detected when the patient is using nicotine replacement therapy patches and/or gum.  Use of nicotine replacement therapy products must be discontinued for 7 - 10 days in order for the nicotine and cotinine to no longer be detected. 

Additional Information:

Both nicotine and cotinine values will be reported.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.