Feb 1, 2018




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Toxicology


Pentobarbital (HCPENT) Toxicology Lab Test

Name: Pentobarbital

Test Name: Pentobarbital

Health Link Test Code: HCPENT

LIS Test Code: PENT

CPT Code(s): 80345

Methodology: Gas Chromatography

Clinical Information:

Hypnotic, sedative

Days Performed: Daily, dayshift- Pathology Resident approval is required after hours.

In-Lab Turnaround Time: 1 day.

Stat In-Lab Turnaround Time: 3 hours.

Specimen: Blood

Collection Container: Red top

Collection Volume: 3 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

14 days

Stability Refridgerated:

14 days

Stability Frozen:

14 days

Sample Analyzed: Serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.4 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Specimens collected in red cap with yellow ring (SST) tubes are not acceptable.


Generally effective therapeutic concentration when used in treatment of increased intracranial pressure:


18 years and up: 2.5 - 4.0 mg/dL


All results greater than 3.0 mg/dL are considered critical and will be called to the ordering source. 


Interpretation Type: Interpretive Guidelines

Critical Calls: yes

Additional Information:

Result of "BELOW DETECTION" indicates that the result is below the method detection limit of 0.2 mg/dL. The half-life is 15-48 hours. Peak serum concentration is usually reached 0.5-2 hours after a single oral dose.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories.  This test meets the standards for clinical testing.  This test should not be regarded as investigational or for research use.  The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.