Test Name: Gentamicin
Health Link Test Code: HCGENT
LIS Test Code: GENT
CPT Code(s): 80170
Methodology: Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)
Days Performed: Daily, 24 hours.
In-Lab Turnaround Time: 4 hours.
Stat In-Lab Turnaround Time: 1 hour.
Collection Container: Red top
Also Acceptable: Green top (with lithium heparin anticoagulant)
Collect specimen for peak level 30 minutes after the end of a 30 or 60 minute infusion. Collect specimen for trough level 15 - 30 minutes prior to start of infusion.
Collection Volume: 2 mL
Pediatric Collection Volume: 0.4 mL
7 days off cells
Sample Analyzed: Serum or plasma
Testing Volume: 1 mL
Pediatric Testing Volume: 0.2 mL
Centrifuge. Transfer cell-free serum or plasma to plastic vial. Refrigerate.
Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.
Specimens collected in red cap with yellow ring (SST) or green cap with yellow ring (PST) tubes are not acceptable.
|Optimal therapeutic ranges:|
Sisomicin cross-reacts with this method. Therefore, method cannot reliably be used for patients receiving this antibiotic in combination with gentamicin.
Critical Calls: yes
Result of "BELOW" indicates that the result is below the method detection limit of 0.5 ug/mL. Gentamicin is an aminoglycoside antibiotic which exhibits high potency and broad spectrum antibacterial action against both gram-negative and gram-positive organisms. Patient samples which contain the drugs Sisomycin and Netilomycin will yield falsely elevated values for Gentamicin.