Feb 8, 2018




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Toxicology


Gentamicin (HCGENT) Toxicology Lab Test

Name: Gentamicin

Test Name: Gentamicin

Health Link Test Code: HCGENT

LIS Test Code: GENT

CPT Code(s): 80170

Methodology: Particle Enhanced Turbidometric Inhibition Immunoassay (PETINIA)

Clinical Information:


Days Performed: Daily, 24 hours.

In-Lab Turnaround Time: 4 hours.

Stat In-Lab Turnaround Time: 1 hour.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Green top (with lithium heparin anticoagulant)

Collection Instructions:

Collect specimen for peak level 30 minutes after the end of a 30 or 60 minute infusion. Collect specimen for trough level 15 - 30 minutes prior to start of infusion.

Collection Volume: 2 mL

Pediatric Collection Volume: 0.4 mL

Stability Refridgerated:

7 days off cells

Stability Frozen:

14 days

Sample Analyzed: Serum or plasma

Testing Volume: 1 mL

Pediatric Testing Volume: 0.2 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum or plasma to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Specimens collected in red cap with yellow ring (SST) or green cap with yellow ring (PST) tubes are not acceptable.


 Optimal therapeutic ranges:  


5-10 ug/mL


<1-4 ug/mL


>10.0 ug/mL


Test Limitations:

Sisomicin cross-reacts with this method. Therefore, method cannot reliably be used for patients receiving this antibiotic in combination with gentamicin.

Critical Calls: yes

Additional Information:

Result of "BELOW" indicates that the result is below the method detection limit of 0.5 ug/mL. Gentamicin is an aminoglycoside antibiotic which exhibits high potency and broad spectrum antibacterial action against both gram-negative and gram-positive organisms. Patient samples which contain the drugs Sisomycin and Netilomycin will yield falsely elevated values for Gentamicin.