Apr 6, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Ref Lab- Miscellaneous

Blastomyces Ag

Blastomyces Ag (HCBLSTAG) Ref Lab- Miscellaneous Lab Test

Name: Blastomyces Ag

Test Name: Blastomyces Ag

Health Link Test Code: HCBLSTAG


CPT Code(s): 87449

Methodology: Enzyme Immunoassay

Clinical Information:

May be used as an aid in diagnosis of disseminated or acute pulmonary blastomycosis.


Blastomyces antigen levels decline with therapy and increase with relapse, offering a method to monitor the effect of treatment and diagnose relapse.


This assay detects Blastomyces dermatitidis antigen.

Testing Site: Testing will be sent to an approved reference laboratory

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 1 week.

Collection Instructions:

Specimen Container Minimum Volume
BAL Sterile screw cap container 0.5 mL
Blood 9 mL red top or green top tube 1.2 mL serum
Body Fluid Sterile screw cap containers 0.5 mL

Numbered specimen tube from sterile

dispolsable lumbar puncture tray

0.8 mL
Urine Sterile screw cap container 0.5 mL

Stability Ambient:

7 days

Stability Refridgerated:

30 days

Stability Frozen:


Testing Volume: 4 mL

Pediatric Testing Volume: 2 mL

Specimen Processing:

Blood: Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.


Other specimen types: Refrigerate.

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Samples collected in EDTA-lavender top tubes.

Test Limitations:

Cross reactions due to closely related antigenic epitopes are seen in patients with histoplasmosis, paracoccidioidomycosis, and penicilliosis. Rare cross reactions have been seen in patients with aspergillosis and cryptococcosis.