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Jun 22, 2015

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Molecular Diagnostics

Herpes Simplex by PCR

Herpes Simplex by PCR (HCHSVPCR) Molecular Diagnostics Lab Test

Name: Herpes Simplex by PCR

Test Name: Herpes Simplex by PCR

Health Link Test Code: HCHSVPCR

LIS Test Code: HSVPCR

CPT Code(s): 87529

Test Component:

Real-time PCR detection and typing for Herpes Simplex Virus (HSV) -1 and HSV-2.

Methodology: Real-Time PCR followed by Melting Point Analysis

Clinical Information:

Detect and genotype HSV-1 and HSV-2.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 2 - 3 days.

Stat In-Lab Turnaround Time: 1 - 2 days.

Specimen: BAL, vesicle swab or fluid, genital lesion swab, mouth swab, biopsy, CSF, body fluid, eye fluid, blood

Collection Container: See collection instructions.

Collection Instructions:

Blood- draw in lavender top tube. Do not spin.

 

Biopsy/Tissue specimens- place in refrigerated M4 transport media, Room temperature M4 (M4RT) media, or UTM (Universal Transport Medium). Do not freeze tissue in medium.

 

CSF, BAL, eye fluid and other body fluids- place in a sterile screw cap container. Do not place fluids in UTM.

 

Vesicle swab, genital swab, mouth swab- place swab in refrigerated M4 transport media, Room temperature M4 (M4RT) media, or UTM (Universal Transport Medium).

 

Obtain UTM from UWHC Central Service.

 

Collect specimen within 3-4 days of onset of symptoms, but not more than 7 days. Calcium alginate, wooden and transport swabs containing gel should not be used. Use rayon or Dacron swabs instead. Do not sample crusted lesions.

Collection Volume: 6 mL

Pediatric Collection Volume: 2 mL

Stability Ambient:

3 days

Stability Refridgerated:

7 days

Stability Frozen:

30 days; Do not freeze tissue or whole blood.

Testing Volume: 3 mL

Pediatric Testing Volume: 1.2 mL

Specimen Processing:

Do not spin whole blood.

Specimen Transport:

UTM or M4RT specimen may be transported at room temperature (tissue and swab only).

Unacceptable Criteria:

Calcium alginate, wooden and transport swabs containing gel should not be used. Dry specimens are not acceptable.

Interpretation:

Expected results: HSV not detected.

 

A negative result does not rule out the presence of PCR inhibitors in the patient specimen or HSV DNA concentrations below the level of detection by the assay. This assay detects and genotypes the virus, i.e. distinguishes between HSV-1 and HSV-2. In unusual cases, due to genotype variations or infection by a combination of more than one virus, a result of "Detected, genotype not determined" may be reported.

 

Interpretation Type: Expected Results

Test Limitations:

False negative results may be due to the suppression of viral replication to levels below the detection threshold, or to inhibitory substances present in the specimen.

Additional Information:

Available on weekends for CSF and ocular specimens only.

 

The performance characteristics of this test were validated by UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test; however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.