Oct 26, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Molecular Diagnostics

EBV Qualitative by PCR, BAL

EBV Qualitative by PCR, BAL (HCEBVBAL) Molecular Diagnostics Lab Test

Name: EBV Qualitative by PCR, BAL

Test Name: EBV Qualitative by PCR, BAL

Health Link Test Code: HCEBVBAL


CPT Code(s): 87798

Methodology: Real-Time PCR

Clinical Information:

Detect Epstein-Barr Virus (EBV) in bronchoalveolar lavage specimens.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 2 - 3 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Bronchoalveolar lavage (BAL)

Collection Container: Sterile screw cap container

Collection Instructions:

10-50 mL of lavage specimen is recommended.

Collection Volume: 50 mL

Pediatric Collection Volume: 12 mL

Stability Ambient:

3 days

Stability Refridgerated:

3 days

Stability Frozen:

30 days

Sample Analyzed: Bronchoalveolar lavage (BAL) fluid

Testing Volume: 3 mL

Pediatric Testing Volume: 1.2 mL

Specimen Transport:

Transport specimen to Laboratory immediately. Transport fluid on coolant pack.

Unacceptable Criteria:

LIMIT: One specimen every 48 hours.


Not detected


Detection of low levels of EBV in specimens containing leukocytes is not unexpected, due to the ubiquitous nature of EBV infection in the population.

Interpretation Type: Expected Results

Additional Information:

False negative results may be due to the suppression of viral replication to levels below the detection threshold, insufficient specimen collection volumes, high dilution volumes, or to inhibitory substances that may be present in the specimen. Inadequate specimen collection, processing and storage may invalidate test results. This test should not be used as the only criterion to form a clinical conclusion, instead, results should be correlated with other test results, patient symptoms and clinical presentation. DNA sequence polymorphisms in EBV strains may results in underestimation of viral levels. The presence of variations in EBV DNA sequences are monitored in the real-time PCR assay. This assay does not genotype the virus, and consequently does not provide any information with regard to EBV drug resistance.


The performance characteristics of this test were validated by UWHC Clinical Laboratories. The U.S. Food and Drug Administration (FDA) has not approved or cleared this test; however, FDA approval or clearance is currently not required for clinical use of this test. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. The UWHC Clinical Laboratories is authorized under Clinical Laboratory Improvement Amendments (CLIA) to perform high-complexity testing.