Jun 12, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Molecular Diagnostics

Factor V Leiden Gene Mutation Analysis

Factor V Leiden Gene Mutation Analysis (XFAC5) Molecular Diagnostics Lab Test

Name: Factor V Leiden Gene Mutation Analysis

Test Name: Factor V Leiden Gene Mutation Analysis

Health Link Test Code: XFAC5

LIS Test Code: FVPCR

CPT Code(s): 81241

Test Component:

Factor V  Leiden gene mutation analysis by real-time PCR amplification and melting point analysis

Methodology: Real-Time PCR followed by Melting Point Analysis

Clinical Information:

Factor V Leiden Gene Mutation Analysis is useful for confirming the diagnosis of resistance to APC as identified by functional coagulation testing. It can also be used to establish the diagnosis of resistance to APC in patients in whom functional coagulation testing may not be feasible, for example, patients on anticoagulant therapy (especially heparin) or positive for Lupus anticoagulants. Usually ordered with the Factor V is a test for the Prothrombin G20210A allele, which has been associated with elevated plasma prothrombin levels and moderately increased risk of first venous thrombotic events.

Days Performed: Once a week.

In-Lab Turnaround Time: 10 days.

Specimen: Blood

Collection Container: Lavender top

Also Acceptable: Light blue top (3.2% NaCitrate)

Collection Instructions:

For UWMF - ordering source must fill out the UWMF Genetic Lab Request prior to specimen collection.

Collection Volume: 4 mL

Pediatric Collection Volume: 2 mL

Stability Ambient:

7 days

Stability Refridgerated:

3 weeks

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 2 mL

Pediatric Testing Volume: 1.2 mL

Specimen Transport:

Transport specimen to Laboratory immediately. Refrigerate specimen if not delivered immediately. Transport specimen with coolant pack if coming from clinic location; avoid freezing. Specimen must be received within 7 days of collection date.


A written interpretive report is provided by the laboratory.

Additional Information:

This test was performed using a modification of an FDA approved method. The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for clinical testing.


A professional fee is associated with this test.