Name: Factor V Leiden Gene Mutation Analysis
Test Name: Factor V Leiden Gene Mutation Analysis
Health Link Test Code: XFAC5
LIS Test Code: FVPCR
CPT Code(s): 81241
Factor V Leiden gene mutation analysis by real-time PCR amplification and melting point analysis
Methodology: Real-Time PCR followed by Melting Point Analysis
Factor V Leiden Gene Mutation Analysis is useful for confirming the diagnosis of resistance to APC as identified by functional coagulation testing. It can also be used to establish the diagnosis of resistance to APC in patients in whom functional coagulation testing may not be feasible, for example, patients on anticoagulant therapy (especially heparin) or positive for Lupus anticoagulants. Usually ordered with the Factor V is a test for the Prothrombin G20210A allele, which has been associated with elevated plasma prothrombin levels and moderately increased risk of first venous thrombotic events.
Days Performed: Once a week.
In-Lab Turnaround Time: 10 days.
Collection Container: Lavender top
Also Acceptable: Light blue top (3.2% NaCitrate)
For UWMF - ordering source must fill out the UWMF Genetic Lab Request prior to specimen collection.
Collection Volume: 4 mL
Pediatric Collection Volume: 2 mL
Sample Analyzed: Whole Blood
Testing Volume: 2 mL
Pediatric Testing Volume: 1.2 mL
Transport specimen to Laboratory immediately. Refrigerate specimen if not delivered immediately. Transport specimen with coolant pack if coming from clinic location; avoid freezing. Specimen must be received within 7 days of collection date.
A written interpretive report is provided by the laboratory.
This test was performed using a modification of an FDA approved method. The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for clinical testing.
A professional fee is associated with this test.