Name: HIV-1 RNA, Quantitative (Viral Load)
Test Name: HIV-1 RNA, Quantitative (Viral Load)
Health Link Test Code: XHIV1
LIS Test Code: HIVRNA
CPT Code(s): 87536
This test is not used for the diagnosis for HIV-1 infection. This test is used to determine viral burden in known HIV-1 positive patients.
Please contact clinical labs (608-263-7060) prior to adding on to make sure there is a usable specimen available.
Days Performed: Twice a week-days vary.
In-Lab Turnaround Time: 5 days.
Stat In-Lab Turnaround Time: Not available stat.
Collection Container: Lavender top
Collection Volume: 6 mL
Pediatric Collection Volume: 2 mL
Whole blood - 24 hours
Plasma - 5 days
|Whole Blood||Not acceptable|
|Plasma||12 weeks at -20 to -80°C|
Sample Analyzed: Plasma
Testing Volume: 3 mL
Pediatric Testing Volume: 1.2 mL
Separate cell-free plasma from whole blood and transfer to a sterile polypropylene tube within 6 hours of collection. Freeze plasma at -60°C to -80°C (preferred). Prior to freezing at -60°C to -80°C, EDTA plasma may also be stored at either 2°C to 8°C for up to 5 days or frozen at -20°C for up to 90 days.
Outreach Specimen Processing:
Transport frozen plasma on dry ice to Laboratory.
Transport specimen to Laboratory immediately.
If HIV-1 is detected, two results are reported for each assay: a measured log10 copies/mL and a calculated copies/mL result.
Analytical measurement range of this assay is 30 - 10,000,000 copies/mL (1.47-7.00 log10 copies/mL). In patients where HIV-1 is detected but outside of the analytical measurement range (AMR), a result of <30 copies/mL or >10,000,000 copies/mL will be reported. If no HIV-1 is detected, a result of "Not detected" will be reported.
Interpretation Type: Interpretive Guidelines
This assay is not a screening, confirmatory or test procedure for the diagnosis of HIV-1 infection.
Though rare, mutations within the highly conserved regions of the viral genome covered by primers and/or probes in the Aptima HIV-1 quant assay may result in underquantification of or failure to detect the virus.
Specimens are assayed using the Aptima HIV-1 Quant assay. This is a quantitative test for the HIV-1 RNA virus groups M, N and O.
This test is approved by the U.S. Food and Drug Administration.