Mar 24, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Microbiology

H. pylori by CLO Test

H. pylori by CLO Test (HCCLO) Microbiology Lab Test

Name: H. pylori by CLO Test

Test Name: H. pylori by CLO Test

Health Link Test Code: HCCLO

LIS Test Code: CLO

CPT Code(s): 87077

Methodology: Enzymatic

Clinical Information:

The H. pylori CLO test detects the urease enzyme of Helicobacter pylori in gastric mucosal biopsies. It is intended for the presumptive diagnosis of H. pylori infection.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 1 day.

Stat In-Lab Turnaround Time: Not available stat.

Patient Preparation:

Patients should discontinue the use of antibiotics and bismuth preparations three weeks before the biopsy. These agents may suppress but not eradicate the presence of H. pylori making the organism difficult to detect. Patients should not have ingested proton pump inhibitors two weeks prior to the test as these drugs have been shown to inhibit the growth of H. pylori in some people.

Specimen: Tissue

Collection Container: CLO Test slide

Collection Instructions:

The recommended gastric area to biopsy is at least 2 cm away from the pylorus along the lesser or greater curve of the antrum. Excise tissue that appears normal- avoid tissue that is eroded or ulcerated as H. pylori may be present in smaller numbers around those areas.

Stability Ambient:

72 hours

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Specimen Transport:

Transport CLO test slides to the UWHC Microbiology laboratory at ambient temperature.

Unacceptable Criteria:

1) Biopsies that are too large to be completely imbedded in the CLO test slide.

2) Samples from intestinal metaplasia (H. pylori does not colonize intestinal mucosa).

3) Samples contaminated with formalin.

4) Refrigerated or frozen test slides.



Interpretation Type: Expected Results

Test Limitations:

False negative results may occur due to:


False positive results are rare, but may be due to: