Name: H. pylori by CLO Test
Test Name: H. pylori by CLO Test
Health Link Test Code: HCCLO
LIS Test Code: CLO
CPT Code(s): 87077
The H. pylori CLO test detects the urease enzyme of Helicobacter pylori in gastric mucosal biopsies. It is intended for the presumptive diagnosis of H. pylori infection.
Days Performed: Mon-Fri.
In-Lab Turnaround Time: 1 day.
Stat In-Lab Turnaround Time: Not available stat.
Patients should discontinue the use of antibiotics and bismuth preparations three weeks before the biopsy. These agents may suppress but not eradicate the presence of H. pylori making the organism difficult to detect. Patients should not have ingested proton pump inhibitors two weeks prior to the test as these drugs have been shown to inhibit the growth of H. pylori in some people.
Collection Container: CLO Test slide
The recommended gastric area to biopsy is at least 2 cm away from the pylorus along the lesser or greater curve of the antrum. Excise tissue that appears normal- avoid tissue that is eroded or ulcerated as H. pylori may be present in smaller numbers around those areas.
Transport CLO test slides to the UWHC Microbiology laboratory at ambient temperature.
1) Biopsies that are too large to be completely imbedded in the CLO test slide.
2) Samples from intestinal metaplasia (H. pylori does not colonize intestinal mucosa).
3) Samples contaminated with formalin.
4) Refrigerated or frozen test slides.
Interpretation Type: Expected Results
False negative results may occur due to:
- Recent ingestion of antibiotics, bismuth, proton pump inhibitors or sucralfate.
- Low numbers of H. pylori in tissue sample.
- H. pylori not captured in the biopsied area (patchy distribution).
False positive results are rare, but may be due to:
- Failure to completely imbed the biopsy into the gel which allows contaminating organisms to grow in the tissue.
- Patients who have achlorhydria from bacterial overgrowth (may occur in patients with a history of pernicious anema, gastric surgery, or recent use of proton pump inhibitors).