Jun 28, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Microbiology

Culture, Body Fluid, with Gram Stain

Culture, Body Fluid, with Gram Stain (HCBFC, HCWABF) Microbiology Lab Test

Name: Culture, Body Fluid, with Gram Stain

Test Name: Culture, Body Fluid, with Gram Stain

Health Link Test Code: HCBFC, HCWABF

LIS Test Code: BFC, WABF

CPT Code(s): 87070, 87015, 87205, 87075; Additional CPT codes may be added for processing, identification, and susceptibility testing.

Test Component:

Culture and Gram stain

Methodology: Culture, Microscopy

Days Performed: Daily.

In-Lab Turnaround Time: Preliminary report: 1 day. Final report: Negative culture 5 days; Positive culture 2 days minimum.

Specimen: Body fluid

Collection Container: Sterile screw cap container

Also Acceptable: Bactec® Blood Culture Bottle

Collection Instructions:

Sterile body fluids such as peritoneal, pleural, pericardial, and synovial can be sent in blood culture bottles obtained from UWHC Central Services. Aseptically transfer equal amounts of fluid into aerobic and anaerobic bottles. Send separate aliquot of fluid in a sterile container for Gram stain.


Peritoneal dialysate fluid should NOT be sent in blood culture bottles.  Send 50-100 mLs of dialysate fluid in a sterile container to laboratory. 


Grossly purulent specimens should be sent in a sterile container and not inoculated into blood culture bottles.


Breast milk specimens may be refrigerated for up to 72 hours if not transported immediately to laboratory.


A single specimen is sufficient for performing fungal and aerobic/anaerobic culture.


Send bronchial brush specimens in 1 mL of sterile saline.

Collection Volume: 8 - 20 mL

Stability Ambient:

Sterile Container:  2 hours 

Blood Culture Bottles:  5 hours

Stability Refridgerated:

Not acceptable except for breast milk specimens

Stability Frozen:

Not acceptable

Sample Analyzed: Body fluid aliquot

Testing Volume: 8 - 20 mL

Specimen Transport:

Transport to the laboratory.


No Growth

Interpretation Type: Expected Results

Critical Calls: yes

Additional Information:

Mild to moderate community acquired intraabdominal infections do not need to be cultured.

Complicated community acquired and severe complicated intraabdominal infections as well as immunocompromised patients should be cultured aerobically only. Anaerobic culture is not needed.