Name: Culture, Body Fluid, with Gram Stain
Test Name: Culture, Body Fluid, with Gram Stain
Health Link Test Code: HCBFC, HCWABF
LIS Test Code: BFC, WABF
CPT Code(s): 87070, 87015, 87205, 87075; Additional CPT codes may be added for processing, identification, and susceptibility testing.
Culture and Gram stain
Methodology: Culture, Microscopy
Days Performed: Daily.
In-Lab Turnaround Time: Preliminary report: 1 day. Final report: Negative culture 5 days; Positive culture 2 days minimum.
Specimen: Body fluid
Collection Container: Sterile screw cap container
Also Acceptable: Bactec® Blood Culture Bottle
Sterile body fluids such as peritoneal, pleural, pericardial, and synovial can be sent in blood culture bottles obtained from UWHC Central Services. Aseptically transfer equal amounts of fluid into aerobic and anaerobic bottles. Send separate aliquot of fluid in a sterile container for Gram stain.
Peritoneal dialysate fluid should NOT be sent in blood culture bottles. Send 50-100 mLs of dialysate fluid in a sterile container to laboratory.
Grossly purulent specimens should be sent in a sterile container and not inoculated into blood culture bottles.
Breast milk specimens may be refrigerated for up to 72 hours if not transported immediately to laboratory.
A single specimen is sufficient for performing fungal and aerobic/anaerobic culture.
Send bronchial brush specimens in 1 mL of sterile saline.
Collection Volume: 8 - 20 mL
Sterile Container: 2 hours
Blood Culture Bottles: 5 hours
Not acceptable except for breast milk specimens
Sample Analyzed: Body fluid aliquot
Testing Volume: 8 - 20 mL
Transport to the laboratory.
Interpretation Type: Expected Results
Critical Calls: yes
Mild to moderate community acquired intraabdominal infections do not need to be cultured.
Complicated community acquired and severe complicated intraabdominal infections as well as immunocompromised patients should be cultured aerobically only. Anaerobic culture is not needed.