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Jun 28, 2017

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Chlamydia trachomatis by Amplified Probe Technique

Chlamydia trachomatis by Amplified Probe Technique (HCCTAPT) Microbiology Lab Test

Name: Chlamydia trachomatis by Amplified Probe Technique

Test Name: Chlamydia trachomatis by Amplified Probe Technique

Health Link Test Code: HCCTAPT

LIS Test Code: CTAPT

CPT Code(s): 87491

Methodology: Amplified Probe Technique

Clinical Information:

Detection of Chlamydia trachomatis by Amplified Probe Technique. Concommitant testing for Neisseria gonorrhoeae by Amplified Probe Technique is highly recommended.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Females: Endocervical or vaginal swab or urine. Males: Urethral swab (symptomatic males only) or urine.

Collection Container: APTIMA Collection Kit

Collection Instructions:

Swab Specimens: Obtain room temperature APTIMA Collection Kit from the UWHC Central Services. Follow instructions included with the APTIMA Collection Kit (must use swabs provided).

 

UWHC Central Services Numbers:
Endocervical item #4013141
Male urethral item #4013141
Male and female urine item #4013143
Vaginal item #4013142

Females: Remove and discard any excess mucus, blood, or examination lubricants prior to collection of cervical specimen.

 

Male Urethral swabs: Patient should not urinate for at least 1 hour prior to collection of specimen.

 

Insert unisex swab into the cervix or urethra and rotate 10-30 seconds. Place swab in the APTIMA Collection Kit tube and close cap. Do NOT place large white cleaning swab in APTIMA Collection Kit tube.

 

Specimen may be stored at 2-30°C for up to 7 days.

 

Urine Specimens: Patient should not urinate for at least 1 hour prior to collection of urine specimen. Collect the first 10-50 mL of first catch urine into polypropylene container without preservatives. Female patients should not cleanse the labial area prior to providing the specimen. Midstream urine or >50 mL may increase the chance of false negative results.

 

Urine specimens should be transferred to the APTIMA urine collection kit or sent in a sterile urine container. Urine specimens must be transferred to the kit within 24 hours of collection. APTIMA urine transport tubes must be filled between the two indicator lines on the tube.

 

Urine specimens not in APTIMA Urine Specimen Transport are stable for 24 hours at 2-30°C. Specimens stored in the APTIMA Urine Specimen Transport are stable for 7 days at 2-30°C.

Stability Ambient:

See collection instructions

Stability Refridgerated:

See collection instructions

Stability Frozen:

Not Acceptable

Specimen Transport:

Transport specimen to the laboratory as soon as possible.

Unacceptable Criteria:

Swab must remain in the APTIMA Collection Kit or specimen is unacceptable and must be recollected. Female urethral, wooden swabs, or swabs with aluminum shafts are also unacceptable.

 

APTIMA Collection Kit Tubes containing both unisex swab and large white cleaning swab or large white cleaning swab only are unacceptable.

 

Catheter-collected urine specimens are not acceptable.

 

APTIMA Collection Kits that have been punctured.

 

Urine specimens in APTIMA urine transport that are not filled between the two indicator lines on the transport tube.

Interpretation:

Age 0 days and up: Negative

Interpretation Type: Reference Interval

Test Limitations:

Chlamydia trachomatis by Amplified Probe Technique has been validated for female endocervical and vaginal swabs, urethral swabs from symptomatic males, and male and female urine.

 

For reliable test results, follow instructions for proper specimen and transport conditions.

Additional Information:

Conjunctival, pharyngeal and rectal specimens can be sent to an approved reference lab for NAAT (Nucleic Acid Amplication Testing) or sent for culture.  See C. trachomatis by TMA, Eye/Throat/Rectal.  Specimens other than conjunctival, pharyngeal and rectal must be sent for culture.  See Culture, Chlamydia trachomatis

 

Culture should also be used for post-hysterectomy females who lack a cervix. 

 

Repeat sampling for test of cure is generally not recommended.

 

A minimum of 3 weeks must elapse following treatment before repeat testing.

 

This test was performed using a modification of an FDA approved method. The performance characteristics of this test were determined by the UWHC Clinical Laboratories and meet the standards for clinical testing.