Jun 28, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Microbiology

Culture, CSF with Gram stain

Culture, CSF with Gram stain (HCSFC, GM4210) Microbiology Lab Test

Name: Culture, CSF with Gram stain

Test Name: Culture, CSF with Gram stain

Health Link Test Code: HCSFC, GM4210


CPT Code(s): 87070, 87015, 87205, 87075; Additional CPT codes may be added for processing, identification, and susceptibility testing.

Test Component:

Gram stain and culture.

Methodology: Culture, Microscopy

Days Performed: Daily.

In-Lab Turnaround Time: Preliminary report: 24 hours. Final report: Up to 10 days.

Specimen: Cerebrospinal fluid (CSF)

Collection Container: Numbered specimen tube from sterile disposable lumbar puncture tray

Also Acceptable: Sterile screw cap container

Collection Instructions:

Submit tube 2 from lumbar puncture tray, or shunt/ventriculostomy fluid in sterile screw cap container.

Stability Ambient:

2 hours

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Cerebrospinal fluid (CSF) aliquot

Specimen Transport:

Transport specimen to the laboratory within 2 hours of collection.


No growth

Interpretation Type: Expected Results

Critical Calls: yes

Additional Information:

Anaerobic culture may also be ordered on shunt/ventriculostomy fluid if collected in anaerobic transport medium (UWHC Central Services item#22000060) or in sterile syringe if received in lab within 30 minutes of collection.


Anaerobic culture for Proprionibacterium species for ventricular and shunt fluid specimens only. For lumbar puncture specimens order Culture, CSF with Gram Stain.


Turnaround time for negative anaerobic culture specimens is 10 days.