Name: Culture, Urine
Test Name: Culture, Urine
Health Link Test Code: URC , HCURNCS
LIS Test Code: URNC, URNCS
CPT Code(s): 87086, 87088; Additional CPT codes may be added for processing, identification, and susceptibility testing.
Culture; Gram stain if requested.
Methodology: Culture, Microscopy
Isolate and identify pathogenic organisms causing urinary tract infection.
Days Performed: Daily.
In-Lab Turnaround Time: 1 - 2 days.
Stat In-Lab Turnaround Time: Not available stat.
Specimen: Random urine
Collection Container: Sterile screw cap container
Indicate midstream, indwelling cath, single cath, VB series, cystoscopy, or suprapubic aspirate.
Collection Volume: 1 mL
Pediatric Collection Volume: 1 mL
Sample Analyzed: Urine aliquot
Testing Volume: 0.1 mL
Pediatric Testing Volume: 0.1 mL
Refrigerate specimen if transport to laboratory will be greater than 2 hours.
Transport specimen to the laboratory within 2 hours of collection. Transport specimen with coolant pack if over 2 hours.
LIMIT: One specimen every 24 hours.
None (Less than 1000 CFU/mL)
Interpretation Type: Expected Results
Bacteria present in numbers less than 1000 colony forming units (CFU) per mL are not detected by this method.
In general, in clean catch, midstream specimens, colony counts in fresh bladder urine from infected patients show more than 10,000 CFU/mL.
A colony count of more than 10,000 CFU/mL is significant in urine collected via an indwelling catheter, more than 1,000 CFU/mL if collected by single (straight) catheterization.
Regardless of the collection method, if more than two uropathogens are present at greater than 10,000 CFU/mL, only a single, predominating uropathogen will be identified. If no single uropathogen predominates, the report will include a comment stating "No further workup. Pattern of growth suggests poor specimen quality. Suggest appropriate recollection with timely delivery to laboratory if clinically warranted."
Customized protocols are used for diagnosis of prostatitis, ileal conduits, suprapubic aspirates, cystoscopy or when low count UTI (urethral syndrome) is indicated.
Susceptibility tests are performed based on UWHC Microbiology laboratory protocols.