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Nov 3, 2017

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Microbiology

Respiratory Virus Panel with Bordetella,PCR

Respiratory Virus Panel with Bordetella,PCR (RVPBPCR) Microbiology Lab Test

Name: Respiratory Virus Panel with Bordetella,PCR

Test Name: Respiratory Virus Panel with Bordetella,PCR

Health Link Test Code: RVPBPCR

LIS Test Code: RVPBPCR

CPT Code(s): 87633, 87798x3

Methodology: PCR

Clinical Information:

The Respiratory Virus Panel with Bordetella is a mulitplexed qualitative test for simulataneous detection of Influenza A, Influenza A H1, Influenza A H3, Influenza B, Respiratory Syncytial Virus (RSV) A, RSV B, Parainfluenza  Virus (PIV) 1, PIV 2, PIV 3, PIV 4, Human Metapneumovirus, Rhinovirus, Adenovirus, Bordetella parapertussis/bronchisepticaBordetella holmesii, and Bordetella pertussis.  The detection and identification of specific viral and bacterial nucleic acids from individuals exhibiting signs and symptoms of respiratory infection aids in the diagnosis of respiratory infection if used in conjunction with other clinical and epidemiological information.

Days Performed: Daily.

In-Lab Turnaround Time: 1 - 2 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Nasopharyngeal Minitip swab (flocked swab), BAL

Collection Container: UTM(Universal Transport Medium)

Collection Instructions:

Obtain UTM from UWHC Central Services, item #4010148

Collection Volume: 2 mL

Pediatric Collection Volume: 0.5 mL

Stability Ambient:

2 hours

Stability Refridgerated:

48 hours

Stability Frozen:

Not acceptable

Specimen Transport:

Transport specimen to the laboratory on coolant pack.

Unacceptable Criteria:

Swabs other than naspharyngeal flocked swabs are not acceptable.

 

Transport media received without a swab are not acceptable.

 

Refrigerated M4 and room temperature M4RT are not acceptable.

Interpretation:

Negative

Test Limitations:

Proper collection and transport of specimen is of critical importance for proper detection.

 

Due to genetic similarity between rhinovirus and enterovirus, some strains of enterovirus may be detected as rhinovirus.

 

Due to genetic similarity, if Bordetella pertussis is detected, the presence of Bordetella parapertussis/bronchiseptica cannot be determined.

 

Recent vaccination with intranasal influenza vaccine may produce false positive results for influenza A and/or influenza B.

 

A negative result does not exclude the possibility of viral or bacterial infection.  Negative results should not be used as the sole basis for diagnosis, treatment, or other patient management decisions.

Additional Information:

Refer to Nasopharyngeal Swab Collection for Respiratory PCR Testing for proper collection instructions.

Nasopharyngeal Swab Collection for Respiratory PCR Testing

 

Do not order simultaneously with Influenza A and B and RSV by PCR [HCFLURSV] or RSV Detection [HCRSVPCR].

 

This test is most appropriate for transplant and immunocompromised patients.  All others should condsider Influenza A and B and RSV by PCR [HCFLURSV].

 

If testing for only Bordetella species is desired, order Bordetella species (including B. pertussis), PCR [BORDPCR].

 

Positive influenza A, influenza B, Bordetella pertussis, Bordetella parapertussis/bronchiseptica, and Bordetella holmesii results on inpatients, including those from the ED and subsequently admitted, will be called to the ordering source.

 

For BAL specimens:  This test was performed using a modification of an FDA approved method.  The performance characteristics of this test were determined by the UWHC clinical laboratories and meet the standards for clinical testing.