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Jun 28, 2017

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Microbiology

Trichomonas vaginalis by Amplified Probe Technique

Trichomonas vaginalis by Amplified Probe Technique (TVAPT) Microbiology Lab Test

Name: Trichomonas vaginalis by Amplified Probe Technique

Test Name: Trichomonas vaginalis by Amplified Probe Technique

Health Link Test Code: TVAPT

LIS Test Code: TVAPT

CPT Code(s): 87661

Methodology: Amplified Probe Technique

Clinical Information:

Detection of Trichomonas vaginalis by Amplified Probe Technique. For vaginal specimens, also testing for yeast and bacterial vaginosis (gram stain) is highly recommended.

Days Performed: Mon-Sun.

In-Lab Turnaround Time: 1 - 2 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: FEMALES: Endocervical or vaginal swab or urine. MALES: Urine

Collection Container: APTIMA Collection Kit

Collection Instructions:

Swab Specimens: Obtain room temperature APTIMA Collection Kit from the UWHC Central Services. Follow instructions included with the APTIMA Collection Kit (must use swabs provided). 

UWHC Central Services Numbers:
Endocervical item #4013141
Male and female urine item #4013143
Vaginal item #4013142

Females: Remove and discard any excess mucus, blood, or examination lubricants prior to collection of cervical specimen.

 

For endocervical specimens:  Insert unisex swab into the cervix and rotate 10-30 seconds. Place swab in the APTIMA Collection Kit tube and close cap. Do NOT place large white cleaning swab in APTIMA Collection Kit tube.

 

Specimen may be stored at 2-30°C for up to 7 days.

 

Urine Specimens: Patient should not urinate for at least 1 hour prior to collection of urine specimen. Collect the first 10-50 mL of first catch urine into polypropylene container without preservatives. Female patients should not cleanse the labial area prior to providing the specimen. Midstream urine or >50 mL may increase the chance of false negative results.

 

Urine specimens should be transferred to the APTIMA urine collection kit or sent in a sterile urine container. Urine specimens must be transferred to the kit within 24 hours of collection. APTIMA urine transport tubes must be filled between the two indicator lines on the tube.

 

Urine specimens not in APTIMA Urine Specimen Transport are stable for 24 hours at 2-30°C. Specimens stored in the APTIMA Urine Specimen Transport are stable for 7 days at 2-30°C.

Stability Ambient:

See collection instructions

Stability Refridgerated:

See collection instructions

Stability Frozen:

Not Acceptable

Specimen Transport:

Transport specimen to the laboratory as soon as possible.

Unacceptable Criteria:

Swab must remain in the APTIMA Collection Kit or specimen is unacceptable and must be recollected. Wooden swabs or swabs with aluminum shafts are also unacceptable.

 

APTIMA Collection Kit Tubes containing large white cleaning swab are unacceptable.

 

APTIMA Collection Kits that have been punctured.

 

Urine specimens in APTIMA urine specimen transport that are not filled between the two indicator lines on the transport tube.

 

Catheter-collected urine specimens are not acceptable.

 

Interpretation:

Age 0 days and up: Negative

Interpretation Type: Reference Interval

Test Limitations:

A negative result does not preclude a possible infection because results are dependent on adequate specimen collection.  Test results may be affected by target levels below the assay limit of detection.

 

Trichomonas vaginalis by Amplified Probe Technique has been validated for vaginal and endocervical swabs and urine.  All other sample types will be sent to a reference lab for testing.

 

Additional Information:

Repeat sampling for test of cure is generally not recommened.

 

Urethral specimens can be sent out to an approved reference lab for NAAT (Nucleic Acid Amplification Testing).  See Trichomonas vaginalis by TMA, Urethral specimens.