Name: Trichomonas vaginalis by Amplified Probe Technique
Test Name: Trichomonas vaginalis by Amplified Probe Technique
Health Link Test Code: TVAPT
LIS Test Code: TVAPT
CPT Code(s): 87661
Methodology: Amplified Probe Technique
Detection of Trichomonas vaginalis by Amplified Probe Technique. For vaginal specimens, also testing for yeast and bacterial vaginosis (gram stain) is highly recommended.
Days Performed: Mon-Sun.
In-Lab Turnaround Time: 1 - 2 days.
Stat In-Lab Turnaround Time: Not available stat.
Specimen: FEMALES: Endocervical or vaginal swab or urine. MALES: Urine
Collection Container: APTIMA Collection Kit
Swab Specimens: Obtain room temperature APTIMA Collection Kit from the UWHC Central Services. Follow instructions included with the APTIMA Collection Kit (must use swabs provided).
|UWHC Central Services Numbers:|
|Male and female urine||item #4013143|
Females: Remove and discard any excess mucus, blood, or examination lubricants prior to collection of cervical specimen.
For endocervical specimens: Insert unisex swab into the cervix and rotate 10-30 seconds. Place swab in the APTIMA Collection Kit tube and close cap. Do NOT place large white cleaning swab in APTIMA Collection Kit tube.
Specimen may be stored at 2-30°C for up to 7 days.
Urine Specimens: Patient should not urinate for at least 1 hour prior to collection of urine specimen. Collect the first 10-50 mL of first catch urine into polypropylene container without preservatives. Female patients should not cleanse the labial area prior to providing the specimen. Midstream urine or >50 mL may increase the chance of false negative results.
Urine specimens should be transferred to the APTIMA urine collection kit or sent in a sterile urine container. Urine specimens must be transferred to the kit within 24 hours of collection. APTIMA urine transport tubes must be filled between the two indicator lines on the tube.
Urine specimens not in APTIMA Urine Specimen Transport are stable for 24 hours at 2-30°C. Specimens stored in the APTIMA Urine Specimen Transport are stable for 7 days at 2-30°C.
See collection instructions
See collection instructions
Transport specimen to the laboratory as soon as possible.
Swab must remain in the APTIMA Collection Kit or specimen is unacceptable and must be recollected. Wooden swabs or swabs with aluminum shafts are also unacceptable.
APTIMA Collection Kit Tubes containing large white cleaning swab are unacceptable.
APTIMA Collection Kits that have been punctured.
Urine specimens in APTIMA urine specimen transport that are not filled between the two indicator lines on the transport tube.
Catheter-collected urine specimens are not acceptable.
Age 0 days and up: Negative
Interpretation Type: Reference Interval
A negative result does not preclude a possible infection because results are dependent on adequate specimen collection. Test results may be affected by target levels below the assay limit of detection.
Trichomonas vaginalis by Amplified Probe Technique has been validated for vaginal and endocervical swabs and urine. All other sample types will be sent to a reference lab for testing.
Repeat sampling for test of cure is generally not recommened.
Urethral specimens can be sent out to an approved reference lab for NAAT (Nucleic Acid Amplification Testing). See Trichomonas vaginalis by TMA, Urethral specimens.