Name: Mono Test
Test Name: Mono Test
Health Link Test Code: MSP
LIS Test Code: MONO
CPT Code(s): 86308
Methodology: Immunochromatographic Assay
Days Performed: Daily, 24 hours.
In-Lab Turnaround Time: 4 hours.
Stat In-Lab Turnaround Time: 1 hour.
Collection Container: Red top
Also Acceptable: Lavender top, Red cap with yellow ring (SST)
Collection Volume: 1 mL
Pediatric Collection Volume: 0.5 mL
1 year; avoid repeat freeze/thaw cycles
Sample Analyzed: Serum or plasma
Testing Volume: 0.5 mL
Pediatric Testing Volume: 0.25 mL
Centrifuge. Transfer cell-free serum/plasma to plastic vial. Refrigerate.
Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.
Interpretation Type: Expected Results
NEGATIVE: Heterophile antibodies are usually detectable within 3 wk of EBV infection. During the acute phase of illness, they are detectable in 80-85% of IM cases, about 50% in children under 4 years of age. If clinical suspicion persists, testing for IgM to EBV VCA may be warranted.
POSITIVE: A positive test provides support for the diagnosis of IM due to EBV. Note that heterphile antibodies may persist for months or, in 2-3% of patients, more than 1 year following acute EBV infection. The presence of EBV VCA IgM would be confirmatory of current disease.