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Dec 27, 2017

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,KITS

Mono Test

Mono Test (MSP) KITS Lab Test

Name: Mono Test

Test Name: Mono Test

Health Link Test Code: MSP

LIS Test Code: MONO

CPT Code(s): 86308

Methodology: Immunochromatographic Assay

Days Performed: Daily, 24 hours.

In-Lab Turnaround Time: 4 hours.

Stat In-Lab Turnaround Time: 1 hour.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Lavender top, Red cap with yellow ring (SST)

Collection Volume: 1 mL

Pediatric Collection Volume: 0.5 mL

Stability Refridgerated:

2 days

Stability Frozen:

1 year; avoid repeat freeze/thaw cycles

Sample Analyzed: Serum or plasma

Testing Volume: 0.5 mL

Pediatric Testing Volume: 0.25 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum/plasma to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.

Interpretation:

Negative

Interpretation Type: Expected Results

Test Limitations:

NEGATIVE:  Heterophile antibodies are usually detectable within 3 wk of EBV infection. During the acute phase of illness, they are detectable in 80-85% of IM cases, about 50% in children under 4 years of age. If clinical suspicion persists, testing for IgM to EBV VCA may be warranted.


POSITIVE:  A positive test provides support for the diagnosis of IM due to EBV. Note that heterphile antibodies may persist for months or, in 2-3% of patients, more than 1 year following acute EBV infection. The presence of EBV VCA IgM would be confirmatory of current disease.