Name: HIV Ab/Ag Combo with reflex to HIV 1/2 Differentiation for Confirmation
Test Name: HIV Ab/Ag Combo with reflex to HIV 1/2 Differentiation for Confirmation
Health Link Test Code: HIVABAG
LIS Test Code: HIVABAG
CPT Code(s): 87389 or G0475
Methodology: Chemiluminescent Immunoassay
Detects human immunodificiency virus (HIV) p24 antigen and antibodies to HIV type 1 (HIV-1 group M and group O) and/or type 2 (HIV-2) in human serum.
Days Performed: Daily, 24 hours.
In-Lab Turnaround Time: 4 hours.
Stat In-Lab Turnaround Time: 2 hours.
Collection Container: Red top
Also Acceptable: Red cap with yellow ring (SST)
Collection Volume: 4 mL
Pediatric Collection Volume: 3 mL
Sample Analyzed: Serum
Testing Volume: 2 mL
Pediatric Testing Volume: 1.5 mL
Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.
Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.
Expected results: Nonreactive
Nonreactive: The fourth-generation ELISA screen test is for the simultaneous qualitative detection of HIV Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 and HIV Type 2. Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody. If very early infection is suspected (p24 antigen positive and prior to the development of antibodies), confirmation by HIV PCR will be more sensitive than the Multispot HIV1/2 differentiation assay.
Reactive specimens automatically reflex to the HIV 1/2 Differentiation for Confirmation (HIVDC) test.
Interpretation Type: Interpretive Guidelines
Effective Date of Reference Range: Mon, 10 Aug 2015 00:00:00 CDT
The performance of this assay has not been established with cadaver specimen, heat inactivated specimens or body fluids other than serum.
It is recognized that currently available assays for the detection of antibodies to HIV-1 and/or HIV-2 may not detect all infected individuals. A negative test result does not exclude the possibility of exposure to or infection with HIV. HIV antibodies may be undetectable in some stages of the infection and in some clinical conditions.
Reactive specimens are sent to a reference lab for confirmation.
The DIAGNOSTIC HIV TEST form (SR301399-DT) is required when a UW Health provider submits a test order on a paper lab requisition form.