Mar 19, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

EBV Ab to Nuclear Ag, IgG

EBV Ab to Nuclear Ag, IgG (HCEBVNA) Immunology Lab Test

Name: EBV Ab to Nuclear Ag, IgG

Test Name: EBV Ab to Nuclear Ag, IgG

Health Link Test Code: HCEBVNA

LIS Test Code: EBVNA

CPT Code(s): 86664

Methodology: ELISA

Clinical Information:

The intent of the test system together with other clinical information is to aid in the determination of immune status, and/or aid in the diagnosis of EBV (Epstein-Barr Virus)

Days Performed: Once a week.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red cap with yellow ring (SST)

Also Acceptable: Red top

Collection Volume: 1 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

8 hours

Stability Refridgerated:

7 days

Stability Frozen:

3 months at -20°C or lower

Sample Analyzed: Serum

Testing Volume: 0.5 mL

Pediatric Testing Volume: 0.3 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to the laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Grossly hemolyzed/ lipemic, heat-treated serum, microbially contaminated and serum containing heavily-visible particulate.


Negative: No detectable IgG antibodies to EBNA-1.  Patient is presumed to have no current or previous infecton with EBV and is susceptible to a primary infection.
Equivocal: Immune status cannot be determined.
Positive: Reactive for IgG antibodies to EBNA-1 indicating a past EBV infection. 


All equivocal test results will be repeated within two working days.

Test Limitations:

Patients with severe immunocompomised conditions may show negative results for EBNA antibodies even if antibodies to VCA are present. Likewise, an anti-EBNA response may fail to develop in patients who have an immunodeficiency disease or who are immunosuppressed.


This assay detects antibody to the EBNA-1 antigen and no other EBNA antigens.


The numeric value (OD Ratio) obtained for a reactive specimen is not indicative of the amount of antibody present and cannot be correlated to an antibody titer.