Name: SSB Ab
Test Name: SSB Ab
Health Link Test Code: HCSSB
LIS Test Code: SSB
CPT Code(s): 86235
Included In: ENA panel
Methodology: Enzyme Immunoassay
Antibodies to extractable nuclear antigens (SSA, SSB, RNP, Sm) are found in many connective tissue diseases including SLE (SSA, SSB, RNP, SM), mixed connective tissue disease (high titer RNP), Sjogren's syndrome (SSA, SSB) and scleroderma (SSA, RNP). Sm is very specific for SLE, but not sensitive (30%). Mixed connective tissue disease is almost always (90%) associated with high titer RNP and is associated with antibodies to the 70kd protein by Western blot. SSA is found in approximately 70% of patients with Sjogren's syndrome, whereas SSB is found in 30% of patients. Scleroderma is typically associated with centromere or Scl-70 antibodies, but RNP and SSA are found in a small proportion of patients.
Days Performed: Mon-Fri, dayshift - day varies.
In-Lab Turnaround Time: 1 week.
Collection Container: Red top
Also Acceptable: Red cap with yellow ring (SST)
Collection Volume: 3 mL
Pediatric Collection Volume: 1 mL
No limit at-20°C or lower. Avoid repeat freeze and thaw.
Sample Analyzed: Serum
Testing Volume: 1 mL
Pediatric Testing Volume: 0.3 mL
Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.
Transport specimen to UWHC Hospital Laboratory. Transport with coolant pack if coming from clinic location.
Hemolyzed, grossly lipemic/turbid and icteric specimens are not acceptable.
Normal SSB (0 days and up): < 20
SSB Interpretive Guidelines