/clinical/,/clinical/tools-resources/,/clinical/tools-resources/lab-test-directory/,/clinical/tools-resources/lab-test-directory/immunology/,

/clinical/tools-resources/lab-test-directory/immunology/name-68190-en.labtest

Jul 13, 2017

page

100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

Protein Electrophoresis, reflex to Immunofixation

Protein Electrophoresis, reflex to Immunofixation (HCSPEPR) Immunology Lab Test

Name: Protein Electrophoresis, reflex to Immunofixation

Test Name: Protein Electrophoresis, reflex to Immunofixation

Health Link Test Code: HCSPEPR

LIS Test Code: SPEPR

CPT Code(s): 84165, 84155; if reflexed to immunofixation add 86334

Test Component:

Serum protein electrophoresis and total protein. Reflex to serum immunofixation if applicable.

Methodology: Gel Electrophoresis and Biuret

Clinical Information:

Frequency Recommendations: Unless clinically indicated, frequency to monitor patient is recommended no more than every 30 days. Serum protein electrophoresis is used to evaluate and follow a monoclonal gammopathy. Immunofixation is used to detect and type a monoclonal gammopathy.

Days Performed: Mon-Fri, dayshift - day varies.

In-Lab Turnaround Time: 3 - 5 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Red cap with yellow ring (SST)

Collection Instructions:

If there is the possibility that the patient has cryoprotein, specimens MUST BE DRAWN AND KEPT WARM AT 37°C UNTIL AFTER PROCESSING IS COMPLETE.

 

Before collection, pre-warm a collection tube (e.g., in an infant heel warmer). Have another infant heel warmer on hand to deliver the blood to the laboratory after collection. Keep specimen at 37°C by wrapping a hot pack (infant heel warmer) around the tube immediately after collection. Transport specimen to laboratory within 10 minutes of collection. Contact laboratory if unable to assure delivery within 10 minutes.

Collection Volume: 3 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

Not acceptable

Stability Refridgerated:

1 week

Stability Frozen:

3 months at -20°C. Note: Avoid repeat freeze and thaw.

Sample Analyzed: Serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.2 mL

Specimen Processing:

Centrifuge and transfer cell-free serum to plastic vial. Refrigerate.

 

IMPORTANT: IF THE PATIENT HAS A CRYOPROTEIN, keep the blood specimen at 37°C during entire processing procedure. Place collection tube in the 37°C incubator for two hours. Spin tube at 1730 G-force for 10 minutes at 37°C. Remove spun serum to labeled tube. Put WARM sticker on tube and refrigerate.

Outreach Specimen Processing:

IF THE PATIENT HAS A CRYOPROTEIN, specimen MUST be collected and processed using the Collection and Specimen Processing procedures above prior to transport. Write WARM on the tube and transport specimen with coolant pack after processing is complete.

Specimen Transport:

Transport speciment to Laboratory. Transport with coolant pack if coming from clinic location.

 

IF THE PATIENT HAS A CRYOPROTEIN, transport to the laboratory within 10 minutes of collection. Keep specimen at 37°C wrapping tube in a hot pack (infant heel warmer). Specimen MUST be processed using the Specimen Processing procedure above prior to transport if coming from clinic location. Write WARM on the tube and transport specimen with coolant pack after processing is complete.

Unacceptable Criteria:

Grossly hemolyzed specimens are not acceptable.

Interpretation:

> 1 year and up:

 

 Total protein  6.4-8.3 g/dL
 Albumin  3.86-6.04 g/dL
 Alpha 1

 0.06-0.22 g/dL

 Alpha 2  0.46-0.98 g/dL
 Beta  0.50-1.23 g/dL
 Gamma  0.40-1.28 g/dL
 A:G ratio  1.62-2.72
 M-Spike  0.00-0.00 g/dL

 

 

 

 

 

 

 

 

 

 

 

 

A written interpretive report with the scan is issued as a separate report. 

 

Reference range not available for patients <12 months of age.

Interpretation Type: Reference Interval

Test Limitations:

Protein Electrophoresis with reflex to Immunofixation requests more frequent than 14 days will automatically be cancelled by the laboratory system.

Additional Information:

A professional fee is associated with this test.