Jul 13, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

Immunofixation Electrophoresis, Urine, 24 Hour

Immunofixation Electrophoresis, Urine, 24 Hour (HCUIFIX) Immunology Lab Test

Name: Immunofixation Electrophoresis, Urine, 24 Hour

Test Name: Immunofixation Electrophoresis, Urine, 24 Hour

Health Link Test Code: HCUIFIX

LIS Test Code: UIFIX

CPT Code(s): 86335, 84156

Test Component:

Urine immunofixation and a quantitative urine protein.

Methodology: Gel Electrophoresis

Clinical Information:

Immunofixation is used to test for and classify light chains (Bence Jones protein) in urine. Diseases associated with light chains in urine include gammopathy, amyloidosis, multiple myeloma and other plasma cell dyscrasias.

Days Performed: Mon-Fri, dayshift - day varies.

In-Lab Turnaround Time: 3 - 5 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: 24 hour timed urine

Collection Container: 24 hour urine container

Collection Instructions:

No preservative necessary. For timed urine collections, collect all urine in container provided. Add "Refrigerate" sticker to 24-hour container.


For 24-hour urine test the collection period must be within 22-26 hours. Collection period starts after first void of the period. It is very important to refrigerate specimen during and after the collection. Indicate the date and time collection started and date and time collection finished. If questions about collection process or container does not have instructions attached, call (608) 263-7060 for assistance. Patient instructions available at www.uwhealth.org. Search for 24-hour urine collection or UWH#4339.

Collection Volume: Entire collection

Stability Ambient:

Not acceptable

Stability Refridgerated:

1 week

Stability Frozen:

3 months for concentrated sample

Sample Analyzed: Urine aliquot

Testing Volume: 10 mL

Pediatric Testing Volume: 10 mL

Outreach Specimen Processing:

Mix urine well and note volume and period. Transport 50 mL aliquot on coolant pack to UWHC Hospital Laboratory along with volume and period information.

Specimen Transport:

Immediately after specimen collection is completed, transport specimen to Laboratory. If transport is delayed, refrigerate specimen.

Unacceptable Criteria:

Urines with bloody supernatants are acceptable but not desireable. Urines with preservatives are not acceptable.


A written interpretive report is issued as a separate report.

Test Limitations:

Urine Immunofixation requests more frequent than 14 days will automatically be cancelled by the laboratory system.

Additional Information:

A professional fee is associated with this test.