Name: ANA Screen with Titer if Positive
Test Name: ANA Screen with Titer if Positive
Health Link Test Code: ANAT
LIS Test Code: ANA
CPT Code(s): 86038 - Screen; 86039 - titer (if appropriate)
Fluorescent patterns with titers of anti-nuclear antibodies on all positive samples.
Methodology: Indirect Immunofluorescent Assay
Used to assist the clinician in supporting a diagnosis of connective tissue disease based upon appropriate history and physical examination findings.
Days Performed: Mon-Fri, dayshift.
In-Lab Turnaround Time: 2 days.
Stat In-Lab Turnaround Time: Not available stat.
Collection Container: Red top
Also Acceptable: Red cap with yellow ring (SST)
Collection Volume: 3 mL
Pediatric Collection Volume: 1 mL
No limit at -20° C. Note: Avoid repeat freeze and thaw.
Sample Analyzed: Serum
Testing Volume: 1 mL
Pediatric Testing Volume: 0.2 mL
Centrifuge and transfer cell-free serum to plastic vial. Refrigerate.
Transport specimen to laboratory. Transport with coolant pack if coming from clinic locations.
Grossly hemolyzed and lipemic specimens are not recommended. Body fluids, including CSF, are unacceptable.
0 days and up:
Normal range ANA titer: ≤ 1:80
Pediatric reference intervals were adapted from the literature.
Anti-nuclear antibodies are immunoglobulins directed against cellular antigens. Each serum is titered at 1:40, 1:160 and 1:640. Patterns are reported as homogeneous, speckled, nucleolar, centromere, cytoplasmic and other rare patterns. Negative, 1:40 and 1:80 titers are insignificant levels. A titer of 1:160 is borderline and may be due to exposure to many medications, infections and environmental factors. A low titer ANA result can be seen in many diseases including chronic infections, liver disease and chronic pulmonary disease. Most clinically evident connective tissue diseases (SLE, MCTD, scleroderma, myositis) are associated with high titer (greater than 1:320) ANA results.