Feb 7, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

ANA Screen with Titer if Positive

ANA Screen with Titer if Positive (ANAT) Immunology Lab Test

Name: ANA Screen with Titer if Positive

Test Name: ANA Screen with Titer if Positive

Health Link Test Code: ANAT

LIS Test Code: ANA

CPT Code(s): 86038 - Screen; 86039 - titer (if appropriate)

Test Component:

Fluorescent patterns with titers of anti-nuclear antibodies on all positive samples.

Methodology: Indirect Immunofluorescent Assay

Clinical Information:

Used to assist the clinician in supporting a diagnosis of connective tissue disease based upon appropriate history and physical examination findings.

Days Performed: Mon-Fri, dayshift.

In-Lab Turnaround Time: 2 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: 3 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

Not acceptable

Stability Refridgerated:

7 days

Stability Frozen:

No limit at -20° C. Note: Avoid repeat freeze and thaw.

Sample Analyzed: Serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.2 mL

Specimen Processing:

Centrifuge and transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to laboratory. Transport with coolant pack if coming from clinic locations.

Unacceptable Criteria:

Grossly hemolyzed and lipemic specimens are not recommended. Body fluids, including CSF, are unacceptable.


0 days and up:


Normal range ANA titer: ≤ 1:80


Pediatric reference intervals were adapted from the literature.   

Additional Information:

Anti-nuclear antibodies are immunoglobulins directed against cellular antigens. Each serum is titered at 1:40, 1:160 and 1:640. Patterns are reported as homogeneous, speckled, nucleolar, centromere, cytoplasmic and other rare patterns. Negative, 1:40 and 1:80 titers are insignificant levels. A titer of 1:160 is borderline and may be due to exposure to many medications, infections and environmental factors. A low titer ANA result can be seen in many diseases including chronic infections, liver disease and chronic pulmonary disease. Most clinically evident connective tissue diseases (SLE, MCTD, scleroderma, myositis) are associated with high titer (greater than 1:320) ANA results.