Apr 18, 2017




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

DNA Ab, double-stranded

DNA Ab, double-stranded (DNA) Immunology Lab Test

Name: DNA Ab, double-stranded

Test Name: DNA Ab, double-stranded

Health Link Test Code: DNA

LIS Test Code: ADNA

CPT Code(s): 86225

Methodology: Enzyme Immunoassay

Clinical Information:

Order only if ANA is postive.

DNA antibodies, double-stranded (dsDNA) testing is performed in the evaluation of the patient suspected to have SLE.

 dsDNA antibodies are found in 60% of patients with SLE, most having active disease. Other connective tissue diseases and drug-induced lupus rarely have dsDNA antibodies. Levels of dsDNA antibodies may rise and fall in association with disease activity in SLE (Ref: Arthritis Rheum 33:634-43,1990).

Days Performed: Mon-Fri, dayshift - day varies.

In-Lab Turnaround Time: 1 week.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: 3 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

Not acceptable

Stability Refridgerated:

1 week

Stability Frozen:

4 weeks at -20° C. Note: Avoid repeat freeze and thaw.

Sample Analyzed: Serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.3 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum to clear plastic vial. Refrigerate.

Specimen Transport:

Transport specimen to Laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Hemolyzed, lipemic and turbid specimens are not acceptable.


0 days and up: Negative (0-50 IU/mL)


dsDNA Ab Interpretive Guidelines:


 0-50 IU/mL  Negative
 51-100 IU/mL  Low Positive
 101-200 IU/mL  Moderately Positive
 >200 IU/mL  High Positive



Interpretation Type: Expected Results

Additional Information:

An IFA procedure that uses the parasite Crithidia luciliae to confirm native DNA antibodies, double-stranded in patient sera is available through a reference laboratory upon receipt of request.