Mar 17, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Immunology

Cardiolipin Ab, IgG/IgM

Cardiolipin Ab, IgG/IgM (XCARGM) Immunology Lab Test

Name: Cardiolipin Ab, IgG/IgM

Test Name: Cardiolipin Ab, IgG/IgM

Health Link Test Code: XCARGM

LIS Test Code: ACARD

CPT Code(s): 86147x2

Test Component:

IgG and IgM antibodies to cardiolipin

Methodology: Enzyme Immunoassay

Clinical Information:

Cardiolipin Ab testing is performed for evaluation of hypercoagulable state that may be associated with venous or arterial thromboemboli. Diseases associated with cardiolipin antibodies include SLE, drug-induced lupus, other connective tissue diseases and primary anti-phospholipid antibody syndrome.

Days Performed: Mon-Fri, dayshift - day varies.

In-Lab Turnaround Time: 1 week.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Red top

Also Acceptable: Red cap with yellow ring (SST)

Collection Volume: 3 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

8 hours

Stability Refridgerated:

7 days

Stability Frozen:

No limit at -20° C. Note: Avoid repeat freeze and thaw.

Sample Analyzed: Serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.3 mL

Specimen Processing:

Centrifuge. Transfer cell-free serum to plastic vial. Refrigerate.

Specimen Transport:

Transport serum sample to UWHC Hospital Laboratory. Transport with coolant pack if coming from clinic location.

Unacceptable Criteria:

Grossly hemolyzed, lipemic and turbid specimens are not acceptable.


0 days and up:

IgG:  Negative (0-15 GPL)
IgM:  Negative (0-13 MPL)


Cardiolipin Ab Interpretive Guidelines:

0-15:  Negative 
16-20:  Indeterminate
21-80:  Low-Medium Positive
>80:  Positive 


0-13:  Negative
14-20:  Indeterminate
21-80:  Low-Medium Positive
>80:  Positive

Cardiolipin Ab results are obtained with the INOVA QUANTA Lite ACA IgG and IgM (HRP)ELISA kits.

Interpretation Type: Expected Results

Test Limitations:

Rheumatoid factor, usually an IgM autoantibody, can interfere with the determination of the IgM aCL. Cardiolipin Ab, IgG and IgM values obtained with different manufacturers' assay methods may not be used interchangeably. The magnitude of the reported IgG or IgM levels cannot be correlated to an endpoint titer.