Apr 6, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Histocompatibility

HLA High Resolution, A Locus

HLA High Resolution, A Locus (HCAHIGH) Histocompatibility Lab Test

Name: HLA High Resolution, A Locus

Test Name: HLA High Resolution, A Locus

Health Link Test Code: HCAHIGH


CPT Code(s): 81380

Test Component:

Orderable by locus, but typically for stem cell transplant all HLA loci would be ordered on the recipient and donor: A-locus, B-locus, C-locus, and DRB1-locus.

Methodology: PCR followed by Direct Sequencing

Clinical Information:

High-resolution identification of HLA alleles, typically used for stem cell donor/recipient matching.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 10 days.

Stat In-Lab Turnaround Time: Not available stat.

Patient Preparation:

No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.

Specimen: Blood

Collection Container: Lavender top

Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

7 days

Stability Refridgerated:

3 weeks

Stability Frozen:

Not acceptable

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory.


A written interpretive report will be provided.

Test Limitations:

Identification of HLA alleles generally at the single allele level. Certain combinations may not be resolvable to allele-level identification.

Additional Information:

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.