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Apr 6, 2015

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100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Histocompatibility

HLA KIR Typing

HLA KIR Typing (HCKIRB) Histocompatibility Lab Test

Name: HLA KIR Typing

Test Name: HLA KIR Typing

Health Link Test Code: HCKIRB

LIS Test Code: KIRB

CPT Code(s): 81403

Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)

Clinical Information:

Identifies the presence of KIR (killer cell immunoglobulin-like receptors), some of which may be responsible for binding to HLA ligands and contributing to activation of NK cells. Literature suggests that donor/recipient KIR mismatching may result in lower relapse rate following stem cell transplant.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 5 days.

Stat In-Lab Turnaround Time: Not available stat.

Patient Preparation:

No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.

Specimen: Blood

Collection Container: Lavender top

Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Volume: 4 mL

Pediatric Collection Volume: 2 mL

Stability Ambient:

7 days

Stability Refridgerated:

3 weeks

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 4 mL

Pediatric Testing Volume: 2 mL

Specimen Processing:

Do not centrifuge.

Specimen Transport:

Do NOT open tube. Transport whole blood specimen at room temperature to UWHC Hospital Laboratory.

Interpretation:

An interpretive report is provided by the laboratory, which will report the presence or absence of each of the 16 known KIR genes. A result of "neutral", "bettter", or "best" will be determined according to the below paper, as calculated by the IPD KIR database KIR-B calculator (www.ebi.ac.uk/ipd/kir/donor_b_content.html)

 

Cooley, DJ Weisdorf, LA Guethlein, JP Klein, T Wang, CT Le, SGE Marsh, D Geraghty, S Spellman, MD Haagenson, M Ladner, E Trachtenberg, P Parham and JS Miller. “Donor selection for natural killer cell receptor genes leads to superior survival after unrelated transplantation for acute myelogenous leukemia”. Blood (2010) 116:2411-9.

Additional Information:

Potential donor DNA is stored in the HLA Laboratory for up to 6 months after HLA typing and may be available for KIR analysis. Please check with the lab to verify availability.

 

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.