Name: HLA KIR Typing
Test Name: HLA KIR Typing
Health Link Test Code: HCKIRB
LIS Test Code: KIRB
CPT Code(s): 81403
Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)
Identifies the presence of KIR (killer cell immunoglobulin-like receptors), some of which may be responsible for binding to HLA ligands and contributing to activation of NK cells. Literature suggests that donor/recipient KIR mismatching may result in lower relapse rate following stem cell transplant.
Days Performed: Mon-Fri.
In-Lab Turnaround Time: 5 days.
Stat In-Lab Turnaround Time: Not available stat.
No transfusions (RBC, WBC, Platelets) within 24 hours prior to collection.
Collection Container: Lavender top
Also Acceptable: Yellow top(with ACD-A/B anticoagulant)
Collection Volume: 4 mL
Pediatric Collection Volume: 2 mL
Sample Analyzed: Whole Blood
Testing Volume: 4 mL
Pediatric Testing Volume: 2 mL
Do not centrifuge.
Do NOT open tube. Transport whole blood specimen at room temperature to UWHC Hospital Laboratory.
An interpretive report is provided by the laboratory, which will report the presence or absence of each of the 16 known KIR genes. A result of "neutral", "bettter", or "best" will be determined according to the below paper, as calculated by the IPD KIR database KIR-B calculator (www.ebi.ac.uk/ipd/kir/donor_b_content.html)
Cooley, DJ Weisdorf, LA Guethlein, JP Klein, T Wang, CT Le, SGE Marsh, D Geraghty, S Spellman, MD Haagenson, M Ladner, E Trachtenberg, P Parham and JS Miller. “Donor selection for natural killer cell receptor genes leads to superior survival after unrelated transplantation for acute myelogenous leukemia”. Blood (2010) 116:2411-9.
Potential donor DNA is stored in the HLA Laboratory for up to 6 months after HLA typing and may be available for KIR analysis. Please check with the lab to verify availability.
This test was developed and its performance characteristics determined by this laboratory. It has not been cleared or approved by the US Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.