Apr 6, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Histocompatibility


HLA-B27 (XHLA) Histocompatibility Lab Test

Name: HLA-B27

Test Name: HLA-B27

Health Link Test Code: XHLA

LIS Test Code: B27

CPT Code(s): 81374

Methodology: Reverse SSO detected by Luminex xMAP (Multi-Analyte Profiling)

Clinical Information:

The HLA-B27 antigen is associated with Ankylosing Spondylitis and several other related diseases. The absence of B27 may be used to rule out the associated disease.

Days Performed: Mon-Fri.

In-Lab Turnaround Time: 5 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Also Acceptable: Yellow top(with ACD-A/B anticoagulant)

Collection Instructions:

Do NOT open tube.

Collection Volume: Full 4 mL tube

Pediatric Collection Volume: 2 mL

Stability Ambient:

7 days

Stability Refridgerated:

7 days

Stability Frozen:

Not acceptable

Testing Volume: 4 mL

Pediatric Testing Volume: 2 mL

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory.



 Negative:  HLA-B27 antigen not detected
 Positive:  HLA-B27 antigen detected



Additional Information:

This test was developed and its performance characteristics determined by this laboratory.  It has not been cleared or approved by the US Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.  This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA-88) as qualified to perform high complexity clinical laboratory testing.