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Dec 5, 2016

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Differential without WBC

Differential without WBC (DIFF) Hematology Lab Test

Name: Differential without WBC

Test Name: Differential without WBC

Health Link Test Code: DIFF

LIS Test Code: D1

CPT Code(s): 85004

Test Component:

WBC Differential, RBC morphology and platelet evaluation.
Use of manual microscopy for WBC differential is determined by laboratory personnel during testing.

Included In: CBC with Differential, WBC and Differential

Methodology: Flow Cytometry, Impedance, and where applicable, Manual Microscopy

Days Performed: Daily, 24 hours- all ER specimens performed stat and General Peds/Adolescent Med (GPAM) specimens performed stat upon request (please mark on request form).

In-Lab Turnaround Time: 4 hours.

Stat In-Lab Turnaround Time: 1 hour.

Specimen: Blood

Collection Container: K3EDTA-Lavender top

Also Acceptable: K2EDTA-Lavender top, Pink top tube

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

8 hours

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.25 mL in microtainer

Specimen Transport:

Transport specimen to laboratory at ambient temperature.

Unacceptable Criteria:

Clotted specimens are not acceptable. Tubes with <25% of fill volume.

Interpretation:

Note:  Reference Intervals for relative percent (%) can be found on the patient report.

 

 Age 18 years and up:   
 WBC Neuts Lymphs  
 K/uL cells/uL cells/uL  
 3.8-10.5  1700-7500 1000-3500  
       
       
 Monos Eos Basos  
 cells/uL cells/uL cells/uL  
 200-900 0-500 0-200  
       
 Pediatric Reference Interval*    
 Age  WBC  Neutrophils  Lymphocytes
   K/uL  cells/uL  cells/uL
 0-29 days   9.1-34.0  6000-23500  2500-10500

 30 days-23

     months 

 6.0-14.0  1100-6600  1800-9000
 2-9 years    4.0-12.0  1400-6600  1000-5500
 10-17 years    4.0-10.5  1500-6600  1000-3500
       
 Age  Monocytes  Eosinophils  Basophils
   cells/uL  cells/uL  cells/uL
 0-29 days    < 3500  < 2000  < 400

 30 days-23

     months  

 < 1000  < 700  < 100
 2-9 years    < 1000  < 700  < 100
 10-17 years    < 1000  < 700  < 100

           *from Children's Hospital of Buffalo, 1995/Verified by Milwaukee Children's Hospital

Age Immature Granulocytes
  cells/uL
0-2 days <1700
3 days - 3 months <700
4 months - 10 years <30
>10 years <50

 

Interpretation Type: Reference Interval

Critical Calls: yes

Additional Information:

Differential results may be delayed if a hematopathologist review is warranted. A professional fee may be associated with this test.