Mar 29, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, CD19 and CD20

Flow Cytometry, CD19 and CD20 (HCCD1920) Flow Cytometry Lab Test

Name: Flow Cytometry, CD19 and CD20

Test Name: Flow Cytometry, CD19 and CD20

Health Link Test Code: HCCD1920

LIS Test Code: CD1920

CPT Code(s): 86355, 86356

Test Component:

B-Cell Antigens- CD19,CD20

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

Outside clients should send WBC and Differential results with specimen. If no results accompany request, a WBC and Differential will be added (see transport and limitations for more information)

Collection Volume: 4 mL

Pediatric Collection Volume: 2.5 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 2 mL

Pediatric Testing Volume: 1 - 2 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. For clinic locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Specimens older than 24 hours at the time of analysis are not acceptable.


 Age 18 years and up                     

    %   absolute number/uL
CD3-CD19+   6-24   77-427
CD20   5-16   60-280


Please contact UWHC Flow Cytometry Laboratory at (608) 263-3868 for pediatric reference intervals.

Interpretation Type: Reference Interval

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.