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Jul 31, 2017

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, CAMPTH

Flow Cytometry, CAMPTH (HCCAMPTH) Flow Cytometry Lab Test

Name: Flow Cytometry, CAMPTH

Test Name: Flow Cytometry, CAMPTH

Health Link Test Code: HCCAMPTH

LIS Test Code: CAMPTH

CPT Code(s): 86355, 86359, 86360

Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3+CD4+ (T-helper/inducer cells), CD3+CD8+ (T-cytotoxic/suppressor cells), CD20 (B-cells), CD4:CD8 ratio

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

An order for a WBC and differential must accompany the request. Outside clients should send a WBC and differential results with the specimen. If no results accompany request, a WBC and differential will be added.

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory within 4 hours of collection. Keep specimen at room temperature. DO NOT CENTRIFUGE.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at the time of analysis are not acceptable.

Interpretation:

Age 18 years and up:

   % Absolute number /uL 
 CD3 59-85  767-1903 
 CD3+CD4+ 37-64  481-1464 
 CD3+CD8+ 14-33  247-595 
 CD3-CD20+ 5-16  60-280 
CD4:CD8 1.2-3.7  

 

Please contact UWHC Flow Cytometry Laboratory at (608) 263-3868 for pediatric reference intervals.

Interpretation Type: Reference Interval

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.