Name: Flow Cytometry, Acute Leukemia
Test Name: Flow Cytometry, Acute Leukemia
Health Link Test Code: HCPATHI
LIS Test Code: ALSUB
CPT Code(s): 88184, 88185x24
NOTE: This test is only orderable on Blood specimens. Use Flow Cytometry, Hemepath Consult test (test code FLOWHM/HCPATHI) for all other specimen types.
B-Cell Antigens-CD19, CD20, CD22, cytoplasmic CD79a, T/NK Cell Antigens-CD2, CD3, cytoplasmic CD3, CD4, CD7, CD56, Myeloid Cell Antigens-CD11b, CD13, CD14, CD15, CD16, CD33, CD64, CD117, MPO, Other Antigens-CD10, CD34, CD38, CD45, HLA-DR, TdT
Note: The flow cytometry immunophenotyping panel may be modified by the staff hematopathologist based on sample size and review of morphology and initial flow data.
Methodology: Flow Cytometry
To evaluate the cell surface or cytoplasmic/nuclear antigens present on leukemia or lymphoma cells to determine the lineage of origin and maturation stage. Evaluation of cell morphology (and other relevant testing) is used for interpretation.
Days Performed: Mon-Fri, dayshift- specimens are accepted at any time.
In-Lab Turnaround Time: 2 - 5 days.
Collection Container: Lavender top
Also Acceptable: Green top
Outside clients should send WBC and differential results and an unstained slide along with specimen.
Collection Volume: 4 mL
Pediatric Collection Volume: 2.5 mL
6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.
Sample Analyzed: Whole Blood
Testing Volume: 4 mL
Pediatric Testing Volume: 2.5 mL
Keep specimen at room temperature. Do NOT centrifuge tube.
Transport unopened tube at room temperature to UWHC Hospital Laboratory within 6 hours of collection. Maintain ambient temperature during transport. If sending WBC and differential results, specimen must be received within 8 hours of collection.
Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.
A hematopathologist's written interpretive report is issued as a separate report.
A professional fee is associated with this test.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.