Mar 29, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, Sezary

Flow Cytometry, Sezary (HCPATHI) Flow Cytometry Lab Test

Name: Flow Cytometry, Sezary

Test Name: Flow Cytometry, Sezary

Health Link Test Code: HCPATHI

LIS Test Code: SZP

CPT Code(s): 88184, 88185x9

Test Component:

B-Cell Antigens - CD19, Kappa, Lambda

T/NK Cell Antigens - CD3, CD4, CD5, CD7, CD8

Other Antigens - CD26, CD45

Methodology: Flow Cytometry

Clinical Information:

To evaluate the cell surface or cytoplasmic/nuclear antigens present on leukemia or lymphoma cells to determine the lineage of origin and maturation stage. Evaluation of cell morphology (and other relevant testing) is used for interpretation.

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 2 - 5 days.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

Outside clients should send WBC and differential results and an unstained slide along with specimen.

Collection Volume: 4 mL

Pediatric Collection Volume: 2.5 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 2 mL

Pediatric Testing Volume: 2 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. If sending WBC and differential results, specimen must be received within 8 hours of collection.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.


A Hematopathologist's written interpretive report is issued as a separate report.

Additional Information:

A professional fee is associated with this test.


This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.