Name: Flow Cytometry, Total Lymphocyte Analysis with CD20
Test Name: Flow Cytometry, Total Lymphocyte Analysis with CD20
Health Link Test Code: HCTLA20
LIS Test Code: TLA20
CPT Code(s): 86355, 86357, 86359, 86360, 86356
Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3 CD4 (T-helper/inducer cells), CD3 CD8 (T-cytotoxic/suppressor cells), CD3-CD19+ (B-cells), CD3-CD16 CD56 (NK-cells), CD20 (B-cells), CD4:CD8
Methodology: Flow Cytometry
Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.
In-Lab Turnaround Time: 1 - 4 days.
Stat In-Lab Turnaround Time: Not available stat.
Collection Container: Lavender top
An order for a WBC and differential must accompany request.
Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see transport and limitations for more information).
Collection Volume: 4 mL
Pediatric Collection Volume: 1 mL
6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.
Sample Analyzed: Whole Blood
Testing Volume: 1 mL
Pediatric Testing Volume: 0.5 mL
Keep specimen at room temperature. Do NOT centrifuge tube.
Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.
Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Specimens older than 24 hours at time of analysis are not acceptable.
Age 18 and up:
Please contact UWHC Flow Laboratory at (608) 263-3868 for pediatric reference intervals.
Interpretation Type: Reference Interval
Absolute values will not be calculated if WBC and differential request or results are not received.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.