Mar 29, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, Total Lymphocyte Analysis with CD20

Flow Cytometry, Total Lymphocyte Analysis with CD20 (HCTLA20) Flow Cytometry Lab Test

Name: Flow Cytometry, Total Lymphocyte Analysis with CD20

Test Name: Flow Cytometry, Total Lymphocyte Analysis with CD20

Health Link Test Code: HCTLA20

LIS Test Code: TLA20

CPT Code(s): 86355, 86357, 86359, 86360, 86356

Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3 CD4  (T-helper/inducer cells), CD3 CD8  (T-cytotoxic/suppressor cells), CD3-CD19+  (B-cells), CD3-CD16 CD56  (NK-cells), CD20 (B-cells), CD4:CD8

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

An order for a WBC and differential must accompany request.


Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see transport and limitations for more information).

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Specimens older than 24 hours at time of analysis are not acceptable.


Age 18 and up: 

   %  Absolute number/uL
 CD3:  59-85  767-1903
 CD3+CD4+:  37-64  481-1464
 CD3+CD8+:  14-33  247-595
 CD3-CD19+:  6-24  88-454
 CD3-CD16+CD56+:  6-24  77-427
 CD20:  5-16  60-280
 CD4:CD8 ratio:  1.2-3.7  


Please contact UWHC Flow Laboratory at (608) 263-3868 for pediatric reference intervals.

Interpretation Type: Reference Interval

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.