Name: Flow Cytometry, CD4
Test Name: Flow Cytometry, CD4
Health Link Test Code: HCCD4
LIS Test Code: CD4
CPT Code(s): 86359, 86361
Absolute lymphocyte count, percent and absolute numbers of CD3, CD3 CD4 (T helper/inducer cells)
Methodology: Flow Cytometry
Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.
In-Lab Turnaround Time: 1 - 4 days.
Stat In-Lab Turnaround Time: Not available stat.
Collection Container: Lavender top
An order for a WBC and differential must accompany request.
Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added.
Collection Volume: 4 mL
Pediatric Collection Volume: 1 mL
See Additional Information
Sample Analyzed: Whole Blood
Testing Volume: 1 mL
Pediatric Testing Volume: 0.5 mL
Outreach Specimen Processing:
For BLOOD specimens transport unopened tube at room temperature to UWHC Hospital Laboratory within 6 hours of collection if the WBC and Differential results do not accompany the specimen.
If sending WBC and Differential results with specimen, specimen must be received within 24 hours of collection.
Transport specimen to UWHC Hospital Laboratory within 6 hours of collection. Keep specimen at room temperature. Do NOT centrifuge.
Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.
Age 18 and up:
Please contact UWHC Flow Laboratory at (608) 263-3868 for pediatric reference intervals.
Interpretation Type: Reference Interval
Absolute values will not be calculated if WBC and differential request or results are not received.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use.
The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
Min. 1 mL
Ambient: 8 hours if CBC & Diff is needed
Ambient: 24 hours if CBC & Diff is not needed
Refrigerated: Not acceptable
Frozen: Not acceptable
Transport within 6 hr if cell count is needed
Transport within 22 hr if cell count is not needed