Mar 29, 2015




Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, CD4

Flow Cytometry, CD4 (HCCD4) Flow Cytometry Lab Test

Name: Flow Cytometry, CD4

Test Name: Flow Cytometry, CD4

Health Link Test Code: HCCD4

LIS Test Code: CD4

CPT Code(s): 86359, 86361

Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3, CD3 CD4  (T helper/inducer cells)

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

An order for a WBC and differential must accompany request.


Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added.

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

See Additional Information

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Outreach Specimen Processing:

For BLOOD specimens transport unopened tube at room temperature to UWHC Hospital Laboratory within 6 hours of collection if the WBC and Differential results do not accompany the specimen.


If sending WBC and Differential results with specimen, specimen must be received within 24 hours of collection.

Specimen Transport:

Transport specimen to UWHC Hospital Laboratory within 6 hours of collection. Keep specimen at room temperature. Do NOT centrifuge.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.


Age 18 and up: 


  Absolute number/uL
CD3: 59-85 767-1903
CD3+CD4+: 37-64 494-1464


Please contact UWHC Flow Laboratory at (608) 263-3868 for pediatric reference intervals.

Interpretation Type: Reference Interval

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use.

The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.


Blood Specimens:

Container Volume Stability Transport

Preferred 4mL

Min. 1 mL

Ambient: 8 hours if CBC & Diff is needed

Ambient: 24 hours if CBC & Diff is not needed

Refrigerated: Not acceptable

Frozen: Not acceptable

Transport within 6 hr if cell count is needed


Transport within 22 hr if cell count is not needed