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Mar 29, 2015

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UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, CD4 and CD8

Flow Cytometry, CD4 and CD8 (HCCD48) Flow Cytometry Lab Test

Name: Flow Cytometry, CD4 and CD8

Test Name: Flow Cytometry, CD4 and CD8

Health Link Test Code: HCCD48

LIS Test Code: CD48 (FCD48)

CPT Code(s): 86360, 86359

Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3 (T-cells), CD3 CD4  (T-helper/inducer cells), CD3 CD8  (T-cytotoxic/suppressor cells), CD4:CD8 ratio. (Note: Absolute lymphocyte count and absolute numbers will only be done on blood specimens)

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: See collection instructions.

Collection Instructions:

An order for a WBC and differential must accompany blood request.

 

Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added. See Transport, Limitations and Additional Information for more information.

Stability Ambient:

See additional information

Stability Refridgerated:

See additional information

Stability Frozen:

Not Acceptable

Sample Analyzed: Whole Blood

Outreach Specimen Processing:

For BLOOD specimens transport unopened tube at room temperature to UWHC Hospital Laboratory within 6 hours of collection if the WBC and Differential results do not accompany the specimen.

 

If sending WBC and Differential results with specimen, the specimen must be received within 24 hours of collection.

 

For BAL specimens that have a body fluid cell count ordered the specimen must be transported to UWHC Hospital Laboratory within one hour of collection.

Specimen Transport:

Transport BLOOD specimens to UWHC Hospital Laboratory within 6 hours of collection. Keep specimen at room temperature. Do NOT centrifuge.

 

Transport BAL specimens to UWHC Hospital Laboratory within one hour of collection if a body fluid cell count is ordered.

Unacceptable Criteria:

Blood specimens that are hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Interpretation:

Age 18 and up:

 

   %  Absolute number/uL
 CD3:  59-85  760-1903
 CD3+CD4+:   37-64  494-1464
 CD3+CD8+:  14-33  247-595
 CD4:CD8 ratio:  1.2-3.7  

Please contact UWHC Flow Laboratory at (608) 263-3868 for pediatric reference intervals.

 

No reference ranges for Bronchoalveolar lavage specimens.

Interpretation Type: Reference Interval

Test Limitations:

Absolute values will not be calculated for blood specimens if WBC and differential request or results are not received.

Additional Information:

Blood Specimens:

Container Volume Stability  Transport
Lavender

Preferred: 4 mL

Min. 1 mL

Ambient: 8 hr if CBC & Diff is needed

Ambient: 24 hr if CBC & Diff is not needed

Refrigerate: Not Acceptable

Frozen- Not Acceptable

Transport within 6 hr if cell count needed

 

Transport within 22 hr if cell count not needed

 

BAL Specimens:

Container

Volume Stability  Transport

Sterile

container

Min. 1 mL

Ambient: 2 hr if cell count needed

Ambient: 24 hr if cell count not needed

Refrigerate: 2 hr if cell count needed

Refrigerate: 48 if cell count not needed

Frozen - Not Acceptable

Transport within 1 hr if cell count is needed

 

Transport within 22 hr if cell count not needed

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.