Name: Flow Cytometry, PNH
Test Name: Flow Cytometry, PNH
Health Link Test Code: HCPNH
LIS Test Code: FPNH
CPT Code(s): 88184, 88185x7
Granulocytes that are identified based on CD45 vs Side Scatter (SS) and CD15-positivity are analyzed for GPI anchored proteins, FLAER and CD24. Monocytes that are indentified based on CD45 vs SS and CD64-positivity are analyzed for GPI anchored proteins FLAER and CD14. Red cells expressing CD235a are analyzed for GPI anchored protein CD59.
Methodology: Flow Cytometry
Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.
In-Lab Turnaround Time: 2 - 5 days.
Stat In-Lab Turnaround Time: Not available stat.
Collection Container: Lavender top
An order for a CBC and differential must accompany request. Outside clients should send CBC and differential results along with specimen. If no results accompany request, a CBC and differential will be added (see transport and limitations for more information).
Collection Volume: 4 mL
Pediatric Collection Volume: 1 mL
6 hours if CBC and Differential results do not accompany specimen, 24 hours if CBC and Differential results accompany specimen.
Sample Analyzed: Whole Blood
Testing Volume: 1 mL
Pediatric Testing Volume: 0.5 mL
Keep specimen at room temperature. Do NOT centrifuge tube.
Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport.
Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.
A Hematopathologist's written interpretive report is issued as a separate report.
This test is run by comparing the patient's sample to a "normal control" sample.
A professional fee is associated with this test.
This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.