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Mar 21, 2016

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, Apheresis CD34 (with WBC)

Flow Cytometry, Apheresis CD34 (with WBC) (HCAPCD34) Flow Cytometry Lab Test

Name: Flow Cytometry, Apheresis CD34 (with WBC)

Test Name: Flow Cytometry, Apheresis CD34 (with WBC)

Health Link Test Code: HCAPCD34

LIS Test Code: APCD34

CPT Code(s): 86367, (Stem cell WBC 85048)

Test Component:

Percent and absolute number of CD34 cells in apheresis product and WBC count.

Methodology: Flow Cytometry

Clinical Information:

For determining CD34 level of apheresis collection

Days Performed: Mon-Fri, dayshift.

In-Lab Turnaround Time: 1 day.

Specimen: Apheresis Product

Collection Container: See collection instructions.

Collection Instructions:

Place apheresis product (that is ACD anticoagulated during the apheresis procedure) into a tube containing no anticoagulants. Note: Do not place specimen in a red top tube. Red top tubes contain clot activators. An order for a WBC must accompany request.

Collection Volume: 2.5 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

24 hours

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Apheresis Product

Testing Volume: 2.5 mL

Pediatric Testing Volume: 1 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 16 hours of collection.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.