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Nov 21, 2016

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100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, Immune Deficiency Profile

Flow Cytometry, Immune Deficiency Profile (HCPBPLUS, HCFPPLUS (If WBC and Differential not ordered)) Flow Cytometry Lab Test

Name: Flow Cytometry, Immune Deficiency Profile

Test Name: Flow Cytometry, Immune Deficiency Profile

Health Link Test Code: HCPBPLUS, HCFPPLUS (If WBC and Differential not ordered)

LIS Test Code: PBPLUS, FPPLUS (If WBC and Differential not ordered)

CPT Code(s): 86359, 86355, 86360, 86357, 86356x3, 88184, 88185

Test Component:

Absolute lymphocyte count, total T-cell, B-cell and NK-cell quantitation, CD4 and CD8 subset analysis, CD4:CD8, CD3+CD4+CD45RA+CDRO- (Naive), CD3+CD4+CDRA-CD45RO+(Memory),CD3+ CD4+CD45RA+CD45RO+(Transitional), Total CD3+CD4+CD45RO+ (Memory and Transitional),CD3+TCRalpha/beta+, CD3+TCRgamma/delta+,CD45+CD3+TCRalpha/beta+CD4-CD8-

Methodology: Flow Cytometry

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

An order for a WBC and differential must accompany request.

 

Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added (see transport and limitations for more information).

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. For outreach locations sending WBC and differential results along with specimen, specimen may be received within 8 hours of collection.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Interpretation:

Age 18 and up:

   %   Absolute #/uL
CD3  59-85  767-1903
CD3+CD4+  37-64 481-1464
CD3+CD8+  14-33  247-595
CD3-CD19+  6-24  88-454

CD3-CD16+CD56+

 6-24  77-427
CD3+TCRalpha/beta+ 26.3-84.5

reference interval

not established

CD3+TCRgamma/delta+ 0.2-14.0

reference interval

not established

 CD45+CD3+TCRalpha/beta+CD4-CD8-  <1.5  

reference interval

not established

CD3+CD4+CD45RA+CD45RO-(Naive)

reference interval

not established

reference interval

not established

CD3+CD4+CD4RA+CD45RO+(Transitional)

reference interval

 not established

reference interval

not established

CD3+CD4+CD45RA-CD45RO+(Memory)

reference interval

not established

reference interval

not established

Total CD3+CD4+CD45RO+ (Memory and Transitional)

 

reference interval

not established

 

reference interval

not established

CD4:CD8  1.2-3.7  

 

Please contact UWHC Flow Laboratory at (608) 263-3868 for

pediatric reference intervals.

Interpretation Type: Reference Interval

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.