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Mar 29, 2015

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Flow Cytometry

Flow Cytometry, CD3

Flow Cytometry, CD3 (HCTOT3) Flow Cytometry Lab Test

Name: Flow Cytometry, CD3

Test Name: Flow Cytometry, CD3

Health Link Test Code: HCTOT3

LIS Test Code: TOT3

CPT Code(s): 86359

Test Component:

Absolute lymphocyte count, percent and absolute numbers of CD3 cells (T-cells)

Methodology: Flow Cytometry

Clinical Information:

CD3 analysis is used to follow CD3 level post certain immune suppressive therapies (eg. OKT3, ATG).

Days Performed: Mon-Fri, dayshift- specimens accepted at anytime.

In-Lab Turnaround Time: 1 - 4 days.

Stat In-Lab Turnaround Time: Not available stat.

Specimen: Blood

Collection Container: Lavender top

Collection Instructions:

An order for a WBC and differential must accompany request. Outside clients should send WBC and differential results along with specimen. If no results accompany request, a WBC and differential will be added.

Collection Volume: 4 mL

Pediatric Collection Volume: 1 mL

Stability Ambient:

6 hours if WBC and Differential results do not accompany specimen, 24 hours if WBC and Differential results accompany specimen

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Specimen Processing:

Keep specimen at room temperature. Do NOT centrifuge tube.

Specimen Transport:

Transport unopened tube at room temperature to UWHC Hospital Laboratory within 4 hours of collection. Maintain ambient temperature during transport. DO NOT centrifuge.

Unacceptable Criteria:

Hemolyzed, clotted, frozen or overheated specimens are not acceptable. Samples greater than 24 hours old at time of analysis are not acceptable.

Test Limitations:

Absolute values will not be calculated if WBC and differential request or results are not received.

Additional Information:

This test was developed and its performance characteristics determined by the UWHC Clinical Laboratories. This test meets the standards for clinical testing. This test should not be regarded as investigational or for research use. The US Food and Drug Administration (FDA) has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.