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Nov 3, 2017

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Platelet Reactivity Profile

Platelet Reactivity Profile (Profile order: O183724 (inpatient order), O183723 (ambulatory order)\nIndividual test orders: HCADPAGG, HCVNPRU) Coagulation Lab Test

Name: Platelet Reactivity Profile

Test Name: Platelet Reactivity Profile

Health Link Test Code: Profile order: O183724 (inpatient order), O183723 (ambulatory order) Individual test orders: HCADPAGG, HCVNPRU

LIS Test Code: ADPAGG, VNPRU

CPT Code(s): 85576x2, 85049, 85014

Test Component:

Platelet Reactivity (PRU), Platelet Aggregation ADP, Hematocrit and Platelet Count

Methodology: Impedance

Clinical Information:

Test results are in P2Y12 reaction units (PRU). This test measures the extent of platelet aggregation in the presence of P2Y12 inhibitor drugs such as clopidogrel (Plavix), prasugrel (Effient), ticagrelor (Brilinta) and ticlopidine (Ticlid).

Patient Prep:

Patients who have been treated with Glycoprotein IIb/IIIa inhibitor drugs should not be tested until platelet function has recovered. This time period is approximately 14 days after discontinuation of drug adminstration for abciximab (ReoPro) and up to 48 hours for eptifibatide (Integrilin) and tirofiban (Aggrastat).

Days Performed: Mon-Fri, 0800-1500 - day varies. Specimens will not be accepted prior to 0800 or after 1500. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.

In-Lab Turnaround Time: 2 hours.

Stat In-Lab Turnaround Time: 1 hour.

Patient Preparation:

Patient must be drawn at the following locations only: University Hospital, The American Center, Digestive Health Clinic, University Station, East Clinic and West Clinic.

Specimen: Blood

Collection Container: Light blue top (3.2% Na Citrate) and K3EDTA-Lavender top

Collection Instructions:

Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelet activators. Tube must be allowed to fill completely.

Please indicate patient history and antiplatelet drug and dosage patient is taking.

Collection VNPRU only

Number three patient labels 1 to 3. Collect three 2mL Greiner Bio-One pediatric light blue tubes (white ring). Label each 2 mL light blue pediatric tube in order of draw (first light blue tube collected = 1, second light blue tube collected =2, etc.). You MUST draw the 2 mL Greiner Bio-One pediatric tubes for the Platelet Reactivity  or we will be unable to run the test.

Collection ADPAGG only

Collect one 4 mL light blue tube (black ring) for the ADP aggregation. (Aggregation available M-F 8am-4pm).  Collect one 4 mL lavender tube for the hematocrit and platelet count.

 

Hand Deliver all tubes to UWHC Hospital Laboratory. Tubes sent through the pneumatic tube system will be rejected.


Please call Special Coagulation at 263-5005 with any questions or to obtain a pre-package kit of tubes and instructions.

Collection Volume: 1 full 3.5 mL lt. blue top, 3 full pediatric lt. blue tops filled to mark and 1-4mL lavender top

Pediatric Collection Volume: 3 full pediatric lt. blue tops filled to fill mark and 1-2 mL lavender top

Stability Ambient:

3 hours unspun

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Outreach Specimen Processing:

Do NOT send through the pneumatic tube. Do NOT freeze, refrigerate, or centrifuge. Do NOT open tube. Transport at room temperature. Specimens must be received within 2 hours of collection.

Specimen Transport:

Hand deliver specimen to UWHC Hospital Laboratory ASAP. Do NOT send through a pneumatic tube. Specimens must be received by the laboratory by 1500 Monday through Friday.

 

Unacceptable Criteria:

Hemolyzed or clotted specimens are not acceptable. A partially filled tube is NOT acceptable. Tubes that are not Greiner Bio-One 2mL tubes will be rejected.

Interpretation:

Platelet Reactivity (PRU): Test results are in P2Y12 reaction units (PRU). This measures the extent of platelet aggregation in the presence of P2Y12 inhibitor drugs such as clopidogrel (Plavix), prasugrel (Effient), ticagrelor (Brilinta) and ticlopidine (Ticlid).

Pre-Drug reference range: 194-418 PRU

P2Y12 values <194 (low end of reference range) are specific evidence of a P2Y12 inhibitor effect.

Patients who have been treated with Glycoprotein IIb/IIIa inhibitor drugs should not be tested until platelet function has recovered. This time period is approximately 14 days after discontinuation of drug adminstration for abciximab (ReoPro) and up to 48 hours for eptifibatide (Integrilin) and tirofiban (Aggrastat).

Results should be interpreted in conjunction with other clinical and laboratory data available to the clinician.

 

Platelet Aggregation ADP: 7-36 Ohms

Interpretation Type: Reference Interval