/clinical/,/clinical/tools-resources/,/clinical/tools-resources/lab-test-directory/,/clinical/tools-resources/lab-test-directory/coagulation/,

/clinical/tools-resources/lab-test-directory/coagulation/name-67831-en.labtest

Jun 24, 2016

page

100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Factor X Activity

Factor X Activity (XFAC10) Coagulation Lab Test

Name: Factor X Activity

Test Name: Factor X Activity

Health Link Test Code: XFAC10

LIS Test Code: F10A

CPT Code(s): 85260

Methodology: Photo-Optical Clot Detection

Clinical Information:

Assay used to assess activity of Factor X when clotting abnormality is suspected.

 

NOTE: To monitor Heparin by Anti-Xa units order Heparin level.

Days Performed: Mon-Fri, dayshift - day varies. Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.

In-Lab Turnaround Time: 4 - 10 days.

Stat In-Lab Turnaround Time: 1 day.

Specimen: Blood

Collection Container: Light blue top (3.2% NaCitrate)

Collection Instructions:

Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelets. Tube must be allowed to fill completely.

 

Indicate if patient is receiving anticoagulant (warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy and duration.

Collection Volume: Full 3.5 mL tube

Pediatric Collection Volume: Pediatric tube filled to fill mark

Stability Ambient:

4 hours on a spun tube

Stability Refridgerated:

Not acceptable

Stability Frozen:

2 weeks at -20°C; 6 months at -70°C

Sample Analyzed: Plasma

Testing Volume: 1 mL

Pediatric Testing Volume: 1 mL

Specimen Processing:

Centrifuge specimen to yield platelet poor plasma (platelet count should be less than 10 K/µL). Separate plasma and transfer to plastic tube or vial. Freeze plasma within 4 hours of collection at -20°C or below.

Outreach Specimen Processing:

Centrifuge specimen to yield platelet poor plasma (platelet count should be 10 K/µL or less). Separate plasma and transfer to labeled plastic tube and spin again. Transfer plasma from second spin to clean plastic labeled tube or vial. Indicate on tube "spun twice". Freeze plasma within 4 hours of collection at -20°C or below.

Specimen Transport:

Transport whole blood specimen at room temperature to UWHC Hospital Laboratory within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen plasma aliquot on dry ice to UWHC Hospital Laboratory.

Unacceptable Criteria:

Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.

Interpretation:

 12 months and up:  70.0-150.0% NPP

Factor 10 in normal, full term newborn infants or healthy premature infants may have decreased levels ( > 15-20%) which may not reach adult levels for > 180 days postnatal.

Interpretation Type: Reference Interval

Additional Information:

The Factor X assay is NOT the test for Heparin Level by anti-Xa.