Name: Factor V Activity
Test Name: Factor V Activity
Health Link Test Code: HCF5A
LIS Test Code: F5A
CPT Code(s): 85220
Methodology: Photo-Optical Clot Detection
Assay is used to assess activity level of Factor V when clotting abnormality is suspected. NOTE: To test for Factor V Leiden Gene Mutation order Factor V/Prothrombin Gene Mutation Analysis.
Days Performed: Mon-Fri, dayshift - day varies. Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.
In-Lab Turnaround Time: 4 - 10 days.
Stat In-Lab Turnaround Time: 1 day.
Collection Container: Light blue top (3.2% NaCitrate)
Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelets. Tube must be allowed to fill completely.
Indicate if patient is receiving anticoagulant (warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy and duration.
Collection Volume: Full 3.5 mL tube
Pediatric Collection Volume: Pediatric tube filled to fill mark
4 hours on a spun tube
2 weeks at -20°C; 6 months at -70°C
Sample Analyzed: Plasma
Testing Volume: 1 mL
Pediatric Testing Volume: 1 mL
Centrifuge specimen to yield platelet poor plasma (platelet count should be less than 10 K/µL). Separate plasma and transfer to plastic tube or vial. Freeze plasma within 4 hours of collection at -20°C or below.
Outreach Specimen Processing:
Centrifuge specimen to yield platelet poor plasma (platelet count should be 10 K/µL or less). Separate plasma and transfer to labeled plastic tube and spin again. Transfer plasma from second spin to clean plastic labeled tube or vial. Indicate on tube "spun twice". Freeze plasma within 4 hours of collection at -20°C or below.
Transport whole blood specimen at room temperature to UWHC Hospital Laboratory within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen plasma aliquot on dry ice to UWHC Hospital Laboratory.
Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.
|12 months and up:||60.0-160.0% NPP|
Factor 5 activity in normal, full-term newborn infants may have borderline low or mildly decreased levels (> 30% to 35%) which reach adult levels within 21 days postnatal. Healthy premature infants (30-36 weeks gestation) may have borderline low or mildly decreased levels.
Interpretation Type: Reference Interval
The Factor V assay is NOT the test for Factor V Leiden.