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Oct 7, 2016

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Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Heparin Induced Platelet Ab

Heparin Induced Platelet Ab (HCHEPAB) Coagulation Lab Test

Name: Heparin Induced Platelet Ab

Test Name: Heparin Induced Platelet Ab

Health Link Test Code: HCHEPAB

LIS Test Code: HEPAB

CPT Code(s): 86022

Methodology: ELISA

Clinical Information:

Test is used for the qualitative detection in plasma or serum of the anti-heparin-platelet factor 4 antibodies generated during type II heparin-induced thrombocytopenias.

Days Performed: Daily- Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call (608)263-5005 before collection.

In-Lab Turnaround Time: 2 days.

Stat In-Lab Turnaround Time: 1 day.

Specimen: Blood

Collection Container: Light blue top (3.2% NaCitrate)

Also Acceptable: Red top

Collection Instructions:

Please indicate if patient is receiving anticoagulant (Warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy.

Collection Volume: Full 3.5 mL tube

Pediatric Collection Volume: Pediatric tube filled to fill mark

Stability Ambient:

24 hours on a spun tube

Stability Refridgerated:

Not acceptable

Stability Frozen:

2 weeks at -20°C; 6 months at -70°C

Sample Analyzed: Plasma or serum

Testing Volume: 1 mL

Pediatric Testing Volume: 0.5 mL

Specimen Processing:

Centrifuge specimen to yield platelet poor plasma/serum (platelet count should be less than 10 K/µL). Separate plasma/serum and transfer to plastic tube or vial. Freeze plasma/serum at -20°C or below.

Specimen Transport:

Transport whole blood specimen at room temperature to UWHC Hospital Laboratory within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen aliquot on dry ice to UWHC Hospital Laboratory.

Unacceptable Criteria:

Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.

Interpretation:

0 days and up: Negative

Interpretation Type: Expected Results

Additional Information:

The optical density (OD) of the reference calibrator and the patient are reported as an aid for interpretaton.