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Jul 15, 2016

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100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Protein C Activity

Protein C Activity (XPCF) Coagulation Lab Test

Name: Protein C Activity

Test Name: Protein C Activity

Health Link Test Code: XPCF

LIS Test Code: PC-ACT

CPT Code(s): 85303

Methodology: Chromogenic Assay

Clinical Information:

Test is used for the qualitative determination of plasma Protein C activity by chromogenic method.

Days Performed: Mon-Fri, dayshift - day varies. Specimens are accepted at any time. Testing required during off hours must be approved by a Coagulation physician. Call 263-5005 before collection.

In-Lab Turnaround Time: 1 - 2 weeks.

Stat In-Lab Turnaround Time: 1 day.

Patient Preparation:

The assay is more accurate in the measurement of Protein C in patients taking warfarin, dabigatran, rivaroxaban, edoxaban or apixaban.

Specimen: Blood

Collection Container: Light blue top (3.2% NaCitrate)

Collection Instructions:

Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelets. Tube must be allowed to fill completely.

 

Collection Volume: Full 3.5 mL tube

Pediatric Collection Volume: Pediatric tube filled to fill mark

Stability Ambient:

4 hours on spun tube

Stability Refridgerated:

Not acceptable

Stability Frozen:

1 month at -20°C; 6 months at -70°C

Sample Analyzed: Plasma

Testing Volume: 1.5 mL

Pediatric Testing Volume: 0.5 mL

Specimen Processing:

Centrifuge specimen to yield platelet poor plasma (platelet count should be less than 10 K/uL). Separate plasma and transfer to plastic tube or vial. Freeze plasma within 4 hours of collection at -20°C or below.

Outreach Specimen Processing:

Centrifuge specimen to yield platelet poor plasma (platelet count should be 10 K/µL or less). Separate plasma and transfer to labeled plastic tube and spin again. Transfer plasma from second spin to clean plastic labeled tube or vial. Indicate on tube "spun twice". Freeze plasma within 4 hours of collection at -20°C or below.

Specimen Transport:

Transport whole blood specimen at room temperature to UWHC Hospital Laboratory within 4 hours of collection. See Specimen Processing for instructions if transport criteria are not met. Transport frozen plasma aliquot on dry ice to UWHC Hospital Laboratory.

Unacceptable Criteria:

Hemolyzed specimens, clotted specimens, partially filled tubes and whole blood specimens greater than 4 hours old when received by the laboratory are not acceptable.

Interpretation:

17 years and up: 70- 150 %NPP

 

At birth, Protein C levels are only about 35% of adult normal values on average. Mean Protein C levels rise to above 50% of adult normal values by age 6 months, but may remain below adult normal range until the age of 10-16 years.

Interpretation Type: Reference Interval