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Jan 26, 2018

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100

UWHC,UWMF,

Clinical Hub,Tools & Resources,UWHC Lab Test Directory,Coagulation

Platelet Aggregation, Whole Blood

Platelet Aggregation, Whole Blood (HCWBPA) Coagulation Lab Test

Name: Platelet Aggregation, Whole Blood

Test Name: Platelet Aggregation, Whole Blood

Health Link Test Code: HCWBPA

LIS Test Code: WBPA

CPT Code(s): 85576x4, 823975x5, 85049, 85014

Methodology: Impedance/Luminescence

Clinical Information:

Test measures the ability of platelets to aggregate and/or release in whole blood in response to ADP, arachidonic acid, collagen, thrombin and ristocetin.

Days Performed: Mon-Fri, 0800-1300. Specimens must be RECEIVED by testing lab within 30 minutes of collection if drawn outside of UW Hospital. Call (608) 263-5005 to schedule testing.

In-Lab Turnaround Time: 3 hours.

Patient Preparation:

The results may be affected if the patient has ingested aspirin or any anti-platelet medication, antihistamines, non-narcotic analgesics or NSAIDS prior to testing.

Specimen: Blood

Collection Container: Light blue top (3.2% NaCitrate)

Collection Instructions:

Do NOT draw through a line containing heparin. The venipuncture MUST be atraumatic to avoid hemolysis and contamination with tissue factors and platelet activators. Tube must be allowed to fill completely.

 

Please indicate patient history and if patient is receiving anticoagulant (Warfarin/Heparin/Vitamin K antagonist), direct thrombin inhibitor or fibrinolytic agent therapy and duration.

Collection Volume: Five full 3.5 mL tubes

Pediatric Collection Volume: Six full pediatric tubes filled to fill mark.

Stability Ambient:

Sample must be received in the lab within 30 minutes of the draw.

Stability Refridgerated:

Not acceptable

Stability Frozen:

Not acceptable

Sample Analyzed: Whole Blood

Testing Volume: 15 mL

Pediatric Testing Volume: 10 mL

Specimen Processing:

Do NOT freeze, refrigerate, or centrifuge. Do NOT open tube.

Specimen Transport:

Hand deliver specimen to UWHC Hospital Laboratory ASAP (within 30 mins of the draw). Do NOT send through a pneumatic tube. Specimens must be received by the laboratory by 1230 Monday through Friday.

Unacceptable Criteria:

Hemolyzed or clotted specimens are not acceptable. A partially filled tube is NOT acceptable. Patient ingestion of aspirin for 7 days and/or antihistamines, non-narcotic analgesics, or NSAIDs for 48 hours prior to sample collection is not acceptable.

Interpretation:

 

Component

UOM

Reference Range

Age/Gender

Platelet Count

K/µL

160-370

all ages

Hematocrit

%

44-70

0 days – 29 days

28-55

30 days-5 months

32-42

6 months-23 months

33-43

2-9 years

36-47

10-17 years, male

35-45

10-17 years, female

40-52

18 years and up, male

34-46

18 years and up, female

ADP Aggregation

Ohms

7-36

all ages

ADP Release

nmoles

0.3-1.9

all ages

Arachidonic Acid Aggregation

Ohms

8-36

all ages

Arachidonic Acid Release

nmoles

0.6-2.4

all ages

Thrombin Release

nmoles

>0.5

all ages

1 µg/mL Collagen Aggregation

Ohms

10-33

all ages

1 µg/mL Collagen Release

nmoles

0.5-2.1

all ages

5 µg/mL Collagen Aggregation

Ohms

15-35

all ages

5 µg/mL Collagen Release

nmoles

0.9-2.6

all ages

0.15 mg/mL Ristocetin Aggregation

Ohms

<5

all ages

0.40 mg/mL Ristocetin Aggregation

Ohms

>5

all ages

Spontaneous Aggregation

Ohms

<5

all ages

Interpretation

     

 

Interpretation Type: Reference Interval

Test Limitations:

Platelet count must be greater than 75 K/mcL for test results to be valid.

Additional Information:

An interpretation by the Coagulation Lab Medical Director will be included in the report. A professional fee will be associated with this test.

 

Note: A Platelet Function Screeing test utilizing the Date Behring PFA-100 is also available. See "Platelet Function Screen" or call (608) 263-5005 for information. 

 

Hand deliver sample ASAP (within 30 mins of draw) to UWHC Hospital Laboratory. If unable to deliver, please call UWHC Special Coagulation Lab at (608) 263-5005 and someone will come to pick up the sample.